Anterior cruciate ligament reconstruction in a rabbit model using a silk-collagen scaffold modified by hydroxyapatite at both ends: a histological and biomechanical study

To investigate osteointegration at the graft-bone interface and the prevention of osteoarthritis after anterior cruciate ligament (ACL) reconstruction using a silk-collagen scaffold with both ends modified by hydroxyapatite (HA) in a rabbit model. The HA/silk-collagen scaffold was fabricated using a...

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Bibliographic Details
Published in:Journal of orthopaedic surgery and research 2021-02, Vol.16 (1), p.139-12, Article 139
Main Authors: Bi, Fanggang, Chen, Yangdi, Liu, Junqi, Wang, Yafei, Xu, Danfeng, Tian, Ke
Format: Article
Language:English
Subjects:
Analysis
Anterior cruciate ligament
Anterior cruciate ligament reconstruction
Arthritis
Biocompatibility
Biomechanics
Bone grafts
Bone growth
Bone healing
Collagen
Collagen (type I)
Hydroxyapatite
Immunohistochemistry
Knee
Ligaments
Orthopedic surgery
Orthopedics
Osseointegration
Osteoarthritis
Osteocalcin
Osteointegration
Polyethylene terephthalate
Silk
Tendon-bone healing
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Summary:To investigate osteointegration at the graft-bone interface and the prevention of osteoarthritis after anterior cruciate ligament (ACL) reconstruction using a silk-collagen scaffold with both ends modified by hydroxyapatite (HA) in a rabbit model. The HA/silk-collagen scaffold was fabricated using a degummed, knitted silk scaffold, collagen I matrix, and simulated body fluid (SBF). The HA/silk-collagen scaffold was rolled up to make a graft for replacing the native ACL in the experimental group (HA group), and the silk-collagen scaffold was used in the control (S group). All specimens were harvested at 16 weeks postoperatively to evaluate graft-bone healing and osteoarthritis prevention. Histological staining revealed the massive formation of more mature bone at the tendon-bone interface, and immunohistochemistry staining revealed more collagen I and osteocalcin deposition in the HA group than in the S group. Higher signals indicating more bone mineral formation were detected in the HA group than in the S group, which was consistent with the results of biomechanical testing. Better osteoarthritis prevention was also observed in the HA group, indicating a more stable knee joint in the HA group than in the S group. The HA/silk-collagen scaffold promotes osteointegration at the tendon-bone interface after ACL reconstruction and has great potential for clinical applications.
ISSN:1749-799X
1749-799X
DOI:10.1186/s13018-021-02281-0