Alternate subunit assembly diversifies the function of a bacterial toxin

• Published in: Nature communications 2019-08, Vol.10 (1), p.3684-10, Article 3684
• Main Authors: Fowler, Casey C., Stack, Gabrielle, Jiao, Xuyao, Lara-Tejero, Maria, Galán, Jorge E.
• Format: Article
• Language: English
• Subjects: 13/1; 13/106; 13/31; 14; 14/1; 14/34; 14/63; 45/23; 45/47; 45/70; 631/326/1320; 631/326/41/1319; 631/326/41/2531; 692/420/254; Animals; Bacterial Toxins - genetics; Biological evolution; Cell Line, Tumor; Functional plasticity; Homology; Humanities and Social Sciences; Humans; Laboratory animals; Mice; multidisciplinary; Protein Multimerization - genetics; Protein Subunits - genetics; Regulatory mechanisms (biology); Salmonella; Salmonella typhi - genetics; Science; Science (multidisciplinary); Sequence Homology, Nucleic Acid; Structural design; Structural engineering; Toxins; Typhoid; Versatility; Virulence; Virulence factors; Virulence Factors - genetics; Waterborne diseases
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-019-11592-0

Bacterial toxins with an AB 5 architecture consist of an active (A) subunit inserted into a ring-like platform comprised of five delivery (B) subunits. Salmonella Typhi, the cause of typhoid fever, produces an unusual A 2 B 5 toxin known as typhoid toxin. Here, we report that upon infection of human cells, S . Typhi produces two forms of typhoid toxin that have distinct delivery components but share common active subunits. The two typhoid toxins exhibit different trafficking properties, elicit different effects when administered to laboratory animals, and are expressed using different regulatory mechanisms and in response to distinct metabolic cues. Collectively, these results indicate that the evolution of two typhoid toxin variants has conferred functional versatility to this virulence factor. More broadly, this study reveals a new paradigm in toxin biology and suggests that the evolutionary expansion of AB 5 toxins was likely fueled by the plasticity inherent to their structural design coupled to the functional versatility afforded by the combination of homologous toxin components. Salmonella Typhi produces the typhoid toxin. Here, Fowler et al. show that S . Typhi produces two forms of typhoid toxin that are differentially regulated and display different trafficking properties and different effects when administered to laboratory animals.