An Investigation of an Acute Gastroenteritis Outbreak: Cronobacter sakazakii , a Potential Cause of Food-Borne Illness

• Published in: Frontiers in microbiology 2018-10, Vol.9, p.2549-2549
• Main Authors: Yong, Wei, Guo, Baofu, Shi, Xiaochao, Cheng, Tingting, Chen, Mingming, Jiang, Xiao, Ye, Yanhua, Wang, Junning, Xie, Guoxiang, Ding, Jie
• Format: Article
• Language: English
• Subjects: Cronobacter sakazakii; food-borne illness; gastroenteritis; Microbiology; PFGE; single nucleotide polymorphism; whole genome sequencing
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2018.02549

Whole genome sequencing (WGS) has been widely used in traceability of food-borne outbreaks nowadays. Here, an interesting connection between and food-borne acute gastroenteritis (AGE) was noticed. In October 2016, an AGE outbreak affecting 156 cases occurred in a local senior high school. Case-control study including 70 case-patients and 295 controls indicated a strong association between eating supper at school canteen of the outbreak onset and AGE, as revealed by the Odds Ratio (OR: 95.32). Six recovered strains were evaluated and compared using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and WGS. A phylogenetic tree of whole genomic single nucleotide polymorphisms (wgSNPs) were generated to traceback the potential contamination source in this outbreak. isolates S2 from a patient's rectal swab and S4 from leftover food sample shared identical PFGE pattern and sequence type (ST73), and clustered tightly together in the SNP phylogenetic tree. isolates S5 and S6 from food delivery containers were both ST4 but with different PFGE patterns. isolates S1 and S3 from two patients' rectal swab were sequenced to be and shared another PFGE pattern (ST567). The interesting feature of this study was the implication of as a causative agent in food-borne AGE occurring in healthy adults, although is considered as an opportunistic pathogen and generally affects neonates, infants and immunocompromised adults.