Integrated Analysis of Long Non-Coding RNA and mRNA Expression Profiles in Testes of Calves and Sexually Mature Wandong Bulls (Bos taurus)

The mRNAs and long non-coding RNAs axes are playing a vital role in the regulating of post-transcriptional gene expression. Thereby, elucidating the expression pattern of mRNAs and long non-coding RNAs underlying testis development is crucial. In this study, mRNA and long non-coding RNAs expression...

Full description

Saved in:
Bibliographic Details
Published in:Animals (Basel) 2021-07, Vol.11 (7), p.2006
Main Authors: Liu, Hongyu, Khan, Ibrar Muhammad, Yin, Huiqun, Zhou, Xinqi, Rizwan, Muhammad, Zhuang, Jingyi, Zhang, Yunhai
Format: Article
Language:English
Subjects:
Animal husbandry
Animals
Bioinformatics
Breeding
Calves
Cattle
Cell adhesion
Cell adhesion molecules
Cell cycle
DNA methylation
Enzymes
ErbB-2 protein
Fibroblast growth factor receptor 9
Gene expression
Genes
Genetics
Genomes
Livestock
Livestock production
Lysine
Meat production
Metabolism
Motility
mRNAs and lncRNAs axes
Non-coding RNA
Population genetics
Post-transcription
Sexual maturity
Spermatogenesis
Testes
testicular growth
total RNA-sequencing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The mRNAs and long non-coding RNAs axes are playing a vital role in the regulating of post-transcriptional gene expression. Thereby, elucidating the expression pattern of mRNAs and long non-coding RNAs underlying testis development is crucial. In this study, mRNA and long non-coding RNAs expression profiles were investigated in 3-month-old calves and 3-year-old mature bulls’ testes by total RNA sequencing. Additionally, during the gene level analysis, 21,250 mRNAs and 20,533 long non-coding RNAs were identified. As a result, 7908 long non-coding RNAs (p-adjust < 0.05) and 5122 mRNAs (p-adjust < 0.05) were significantly differentially expressed between the distinct age groups. In addition, gene ontology and biological pathway analyses revealed that the predicted target genes are enriched in the lysine degradation, cell cycle, propanoate metabolism, adherens junction and cell adhesion molecules pathways. Correspondingly, the RT-qPCR validation results showed a strong consistency with the sequencing data. The source genes for the mRNAs (CCDC83, DMRTC2, HSPA2, IQCG, PACRG, SPO11, EHHADH, SPP1, NSD2 and ACTN4) and the long non-coding RNAs (COX7A2, COX6B2, TRIM37, PRM2, INHBA, ERBB4, SDHA, ATP6VOA2, FGF9 and TCF21) were found to be actively associated with bull sexual maturity and spermatogenesis. This study provided a comprehensive catalog of long non-coding RNAs in the bovine testes and also offered useful resources for understanding the differences in sexual development caused by the changes in the mRNA and long non-coding RNA interaction expressions between the immature and mature stages.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani11072006