Multivariate optimization of an ultrasound-assisted extraction procedure for Cu, Mn, Ni and Zn determination in ration to chickens

In this work, multivariate optimization techniques were used to develop a method based on the ultrasound-assisted extraction for copper, manganese, nickel and zinc determination from rations for chicken nutrition using flame atomic absorption spectrometry. The proportions of extracting components (2...

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Published in:Anais da Academia Brasileira de Ciências 2013-09, Vol.85 (3), p.891-902
Main Authors: Barros, Joélia M, Bezerra, Marcos A, Valasques, Gisseli S, Do Nascimento, Jr, Baraquízio B, Souza, Anderson S, De Aragão, Nádia M
Format: Article
Language:English
Subjects:
Animal Feed - analysis
Animals
Chickens
Copper - analysis
extracao assistida por ultrassom
Manganese - analysis
metais
Metals, Heavy - analysis
MULTIDISCIPLINARY SCIENCES
Nickel - analysis
planejamento de misturas
planejamento Doehlert
racoes para frango
Spectrophotometry, Atomic
Ultrasonics
Zinc - analysis
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Summary:In this work, multivariate optimization techniques were used to develop a method based on the ultrasound-assisted extraction for copper, manganese, nickel and zinc determination from rations for chicken nutrition using flame atomic absorption spectrometry. The proportions of extracting components (2.0 mol.L-1 nitric, hydrochloric and acetic acid solutions) were optimized using centroid-simplex mixture design. The optimum proportions of this mixture taken as percentage of each component were respectively 20%, 37% and 43%. Variables of method (sample mass, sonication time and final acid concentration) were optimized using Doehlert design. The optimum values found for these variables were respectively 0.24 g, 18s and 3.6 mol.L-1. The developed method allows copper, manganese, nickel and zinc determination with quantification limits of 2.82; 4.52; 10.7; e 9.69 µg.g-1, and precision expressed as relative standard deviation (%RSD, 25 µg.g-1, N = 5) of 5.30; 2.13; 0.88; and 0.83%, respectively. This method was applied in the analytes determination from chicken rations collected from specialized commerce in Jequié city (Bahia State/Brazil). Application of paired t-test at the obtained results, in a confidence level of 95%, does not show significant difference between the proposed method and the microwave-assisted digestion.
ISSN:0001-3765
1678-2690
1678-2690
0001-3765
DOI:10.1590/S0001-37652013000300005