Detecting Mycoplasma pneumoniae infections in nasopharyngeal specimens from Paediatric patients with asthma exacerbations in Baghdad: A Polymerase Chain Reaction – Gene based study

Numerous viral infections have triggered acute asthma exacerbations. Despite the fact that diagnosis of M. pneumoniae infection is based on sero-prevalence studies but molecular diagnostic techniques, such as PCR, have offered improvements in sensitivity, specificity and rapidity over the latest met...

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Published in:The Egyptian journal of medical human genetics 2018-04, Vol.19 (2), p.117-121
Main Authors: Al-Janabi, Muhi Kadhem Wannas, Ali, Saad Hasan Mohammed, Nasir, Nadia Aziz, Wehby, Muhammed
Format: Article
Language:English
Subjects:
Age
Allergens
Asthma
Atopy
Chest
Children
Consanguinity
Cough
Deoxyribonucleic acid
Dermatitis
DNA
Exacerbation
Family medical history
Females
Fever
Food allergies
Infections
Mycoplasma pneumonia
Mycoplasma pneumoniae
Passive smoking
Pediatrics
Pharynx
Pneumonia
Polymerase Chain Reaction
Rural areas
Seasonal variations
Studies
Viral infections
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creator Al-Janabi, Muhi Kadhem Wannas
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description Numerous viral infections have triggered acute asthma exacerbations. Despite the fact that diagnosis of M. pneumoniae infection is based on sero-prevalence studies but molecular diagnostic techniques, such as PCR, have offered improvements in sensitivity, specificity and rapidity over the latest methods. The aim of this molecular study is to determine the infection rates of M. pneumoniae in acute asthma exacerbation in a group of Iraqi children from Baghdad and also to examine the correlation of the disease with different variable characteristics and symptoms. This study included 94 children between 2 and 13years old; Fifty in-patient asthmatic children and 44 non-asthmatic children as control group who were out-patients of the same hospital. Throat and nasal swab samples were taken for DNA extraction and PCR procedures. PCR results show that 33.3% asthmatic patients were positive for M. pneumoniae while 66.7% were negative (p
doi_str_mv 10.1016/j.ejmhg.2017.08.003
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Despite the fact that diagnosis of M. pneumoniae infection is based on sero-prevalence studies but molecular diagnostic techniques, such as PCR, have offered improvements in sensitivity, specificity and rapidity over the latest methods. The aim of this molecular study is to determine the infection rates of M. pneumoniae in acute asthma exacerbation in a group of Iraqi children from Baghdad and also to examine the correlation of the disease with different variable characteristics and symptoms. This study included 94 children between 2 and 13years old; Fifty in-patient asthmatic children and 44 non-asthmatic children as control group who were out-patients of the same hospital. Throat and nasal swab samples were taken for DNA extraction and PCR procedures. PCR results show that 33.3% asthmatic patients were positive for M. pneumoniae while 66.7% were negative (p&lt;0.001). 53.8% of M. pneumonia-positive asthmatic children were 2–5years while 46.2% were 6–14years old. Among asthmatic patients with positive PCR, 30.8% had positive history of seasonal pattern (p=0.026) and 69.2% have positive family history of atopy (p=0.05). Family history of atopy has strong association with asthma (p=0.005), while factors such as sex, residence, seasonal allergen, animal allergen, passive smoking, mode of delivery or consanguinity has not been associated with asthma. 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subjects Age
Allergens
Asthma
Atopy
Chest
Children
Consanguinity
Cough
Deoxyribonucleic acid
Dermatitis
DNA
Exacerbation
Family medical history
Females
Fever
Food allergies
Infections
Mycoplasma pneumonia
Mycoplasma pneumoniae
Passive smoking
Pediatrics
Pharynx
Pneumonia
Polymerase Chain Reaction
Rural areas
Seasonal variations
Studies
Viral infections
title Detecting Mycoplasma pneumoniae infections in nasopharyngeal specimens from Paediatric patients with asthma exacerbations in Baghdad: A Polymerase Chain Reaction – Gene based study
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