Melanopsin Retinal Ganglion Cells Regulate Cone Photoreceptor Lamination in the Mouse Retina

Newborn neurons follow molecular cues to reach their final destination, but whether early life experience influences lamination remains largely unexplored. As light is among the first stimuli to reach the developing nervous system via intrinsically photosensitive retinal ganglion cells (ipRGCs), we...

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Bibliographic Details
Published in:Cell reports (Cambridge) 2018-05, Vol.23 (8), p.2416-2428
Main Authors: Tufford, Adele R., Onyak, Jessica R., Sondereker, Katelyn B., Lucas, Jasmine A., Earley, Aaron M., Mattar, Pierre, Hattar, Samer, Schmidt, Tiffany M., Renna, Jordan M., Cayouette, Michel
Format: Article
Language:English
Subjects:
cell migration
cone photoreceptors
development
dopamine
intrinsically photosensitive retinal ganglion cells
lamination
melanopsin
mouse
neuronal layers
retina
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Summary:Newborn neurons follow molecular cues to reach their final destination, but whether early life experience influences lamination remains largely unexplored. As light is among the first stimuli to reach the developing nervous system via intrinsically photosensitive retinal ganglion cells (ipRGCs), we asked whether ipRGCs could affect lamination in the developing mouse retina. We show here that ablation of ipRGCs causes cone photoreceptors to mislocalize at different apicobasal positions in the retina. This effect is partly mediated by light-evoked activity in ipRGCs, as dark rearing or silencing of ipRGCs leads a subset of cones to mislocalize. Furthermore, ablation of ipRGCs alters the cone transcriptome and decreases expression of the dopamine receptor D4, while injection of L-DOPA or D4 receptor agonist rescues the displaced cone phenotype observed in dark-reared animals. These results show that early light-mediated activity in ipRGCs influences neuronal lamination and identify ipRGC-elicited dopamine release as a mechanism influencing cone position. [Display omitted] •Light acts via melanopsin retinal ganglion cells to restrict cones to their appropriate layer•Ablation of melanopsin cells changes the cone photoreceptor transcriptome•Dopamine signaling via the D4 receptor is a cue for cone positioning Tufford et al. show that light acting through intrinsically photosensitive retinal ganglion cells (ipRGCs) controls cone photoreceptor positioning in the developing mouse retina, using dopamine signaling as a cue. Ablation of ipRGCs disrupts cone positioning and induces changes in the cone transcriptome.
ISSN:2211-1247
2211-1247
DOI:10.1016/j.celrep.2018.04.086