Natural Molybdenite- and Tyrosinase-Based Amperometric Catechol Biosensor Using Acridine Orange as a Glue, Anchor, and Stabilizer for the Adsorbed Tyrosinase

To develop a natural mineral-based electrochemical enzyme biosensor, natural molybdenite (MLN), tyrosinase (TYR), and acridine orange (AO) were coadsorbed onto a glassy carbon electrode (GCE). The developed TYR/AO/MLN-GCE-based amperometric TYR biosensor exhibited excellent performance for highly se...

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Bibliographic Details
Published in:ACS omega 2021-06, Vol.6 (21), p.13719-13727
Main Authors: Zhang, Yan, Wang, Yue, Zhang, Zhiqiang, Sobhy, Ahmed, Sato, Susumu, Uchida, Masaya, Hasebe, Yasushi
Format: Article
Language:English
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Summary:To develop a natural mineral-based electrochemical enzyme biosensor, natural molybdenite (MLN), tyrosinase (TYR), and acridine orange (AO) were coadsorbed onto a glassy carbon electrode (GCE). The developed TYR/AO/MLN-GCE-based amperometric TYR biosensor exhibited excellent performance for highly sensitive determination of catechol (linear range, 0.1–80 μM; sensitivity, 0.0315 μA/μM; LOD, 0.029 μM; response time,
ISSN:2470-1343
2470-1343
DOI:10.1021/acsomega.1c00973