Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus

We have identified a gene ( PpSAP1 ) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance i...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2017-03, Vol.7 (1), p.332-332, Article 332
Main Authors: Lloret, Alba, Conejero, Ana, Leida, Carmen, Petri, César, Gil-Muñoz, Francisco, Burgos, Lorenzo, Badenes, María Luisa, Ríos, Gabino
Format: Article
Language:English
Subjects:
13/106
38
38/109
38/111
38/77
631/449/2653
631/449/2661/2665
631/449/447
Cell Proliferation
Gene Expression
Gene Expression Regulation, Plant
Hot Temperature
Humanities and Social Sciences
multidisciplinary
Osmotic Pressure
Plant Cells - physiology
Plant Proteins - metabolism
Plants, Genetically Modified
Polyubiquitin - metabolism
Protein Interaction Mapping
Prunus persica - genetics
Prunus persica - physiology
Science
Science (multidisciplinary)
Two-Hybrid System Techniques
Water - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have identified a gene ( PpSAP1 ) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants. PpSAP1 was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release. PpSAP1 expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of PpSAP1 in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as GROWTH-REGULATING FACTOR ( GRF )1-like, TONOPLAST INTRINSIC PROTEIN ( TIP )-like, and TARGET OF RAPAMYCIN ( TOR )-like. Especially, the inverse expression pattern of PpSAP1 and TOR -like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-00471-7