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Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus
We have identified a gene ( PpSAP1 ) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance i...
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| Published in: | Scientific reports 2017-03, Vol.7 (1), p.332-332, Article 332 |
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| creator | Lloret, Alba Conejero, Ana Leida, Carmen Petri, César Gil-Muñoz, Francisco Burgos, Lorenzo Badenes, María Luisa Ríos, Gabino |
| description | We have identified a gene (
PpSAP1
) of
Prunus persica
coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants.
PpSAP1
was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release.
PpSAP1
expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of
PpSAP1
in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as
GROWTH-REGULATING FACTOR
(
GRF
)1-like,
TONOPLAST INTRINSIC PROTEIN
(
TIP
)-like, and
TARGET OF RAPAMYCIN
(
TOR
)-like. Especially, the inverse expression pattern of
PpSAP1
and
TOR
-like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway. |
| doi_str_mv | 10.1038/s41598-017-00471-7 |
| format | article |
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PpSAP1
) of
Prunus persica
coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants.
PpSAP1
was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release.
PpSAP1
expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of
PpSAP1
in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as
GROWTH-REGULATING FACTOR
(
GRF
)1-like,
TONOPLAST INTRINSIC PROTEIN
(
TIP
)-like, and
TARGET OF RAPAMYCIN
(
TOR
)-like. Especially, the inverse expression pattern of
PpSAP1
and
TOR
-like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-017-00471-7</identifier><identifier>PMID: 28336950</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/106 ; 38 ; 38/109 ; 38/111 ; 38/77 ; 631/449/2653 ; 631/449/2661/2665 ; 631/449/447 ; Cell Proliferation ; Gene Expression ; Gene Expression Regulation, Plant ; Hot Temperature ; Humanities and Social Sciences ; multidisciplinary ; Osmotic Pressure ; Plant Cells - physiology ; Plant Proteins - metabolism ; Plants, Genetically Modified ; Polyubiquitin - metabolism ; Protein Interaction Mapping ; Prunus persica - genetics ; Prunus persica - physiology ; Science ; Science (multidisciplinary) ; Two-Hybrid System Techniques ; Water - metabolism</subject><ispartof>Scientific reports, 2017-03, Vol.7 (1), p.332-332, Article 332</ispartof><rights>The Author(s) 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4277-ec69cdae83fc81a88c1cc8fee643639d59c60d3f4440e77ac2f52c411e4f55973</citedby><cites>FETCH-LOGICAL-c4277-ec69cdae83fc81a88c1cc8fee643639d59c60d3f4440e77ac2f52c411e4f55973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428470/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428470/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,37013,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28336950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lloret, Alba</creatorcontrib><creatorcontrib>Conejero, Ana</creatorcontrib><creatorcontrib>Leida, Carmen</creatorcontrib><creatorcontrib>Petri, César</creatorcontrib><creatorcontrib>Gil-Muñoz, Francisco</creatorcontrib><creatorcontrib>Burgos, Lorenzo</creatorcontrib><creatorcontrib>Badenes, María Luisa</creatorcontrib><creatorcontrib>Ríos, Gabino</creatorcontrib><title>Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>We have identified a gene (
PpSAP1
) of
Prunus persica
coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants.
PpSAP1
was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release.
PpSAP1
expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of
PpSAP1
in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as
GROWTH-REGULATING FACTOR
(
GRF
)1-like,
TONOPLAST INTRINSIC PROTEIN
(
TIP
)-like, and
TARGET OF RAPAMYCIN
(
TOR
)-like. Especially, the inverse expression pattern of
PpSAP1
and
TOR
-like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway.</description><subject>13/106</subject><subject>38</subject><subject>38/109</subject><subject>38/111</subject><subject>38/77</subject><subject>631/449/2653</subject><subject>631/449/2661/2665</subject><subject>631/449/447</subject><subject>Cell Proliferation</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Hot Temperature</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Osmotic Pressure</subject><subject>Plant Cells - physiology</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Polyubiquitin - metabolism</subject><subject>Protein Interaction Mapping</subject><subject>Prunus persica - genetics</subject><subject>Prunus persica - physiology</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Two-Hybrid System Techniques</subject><subject>Water - metabolism</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9kU1P3DAQhqOqqCDgD_RQ-UgPaf0ZO5dKiH4hIYFEe7ZmnXHIKmtv7QTEv693QxFc6ovtmXeeseetqveMfmJUmM9ZMtWamjJdUyo1q_Wb6ohTqWouOH_74nxYnea8pmUp3krWvqsOuRGiaRU9qvzXGUaSsJ9HmIYYSPTkASZMJTZh2IcgdMThOJI-xYfpjqweCZA8Jcy5hpyjG0pBR7YpTjgEcnZ7fvOR9BiQlNtNmsOcT6oDD2PG06f9uPr9_duvi5_11fWPy4vzq9pJrnWNrmldB2iEd4aBMY45ZzxiI0Uj2k61rqGd8FJKilqD415xJxlD6ZVqtTiuLhduF2Ftt2nYQHq0EQa7D8TUW0jT4Ea00jfCyTIF0FpygFagbGgjV0wBLZ0L68vC2s6rDXauTCPB-Ar6OhOGO9vHe6skN1LTAjh7AqT4Z8Y82c2Qd4OEgHHOlhnDeMOEkkXKF6lLMeeE_rkNo3bnt138tsVvu_fb7j774eUDn0v-uVsEYhHkkgo9JruOcwrFgP9h_wKpWbZH</recordid><startdate>20170323</startdate><enddate>20170323</enddate><creator>Lloret, Alba</creator><creator>Conejero, Ana</creator><creator>Leida, Carmen</creator><creator>Petri, César</creator><creator>Gil-Muñoz, Francisco</creator><creator>Burgos, Lorenzo</creator><creator>Badenes, María Luisa</creator><creator>Ríos, Gabino</creator><general>Nature Publishing Group UK</general><general>Nature Portfolio</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170323</creationdate><title>Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus</title><author>Lloret, Alba ; Conejero, Ana ; Leida, Carmen ; Petri, César ; Gil-Muñoz, Francisco ; Burgos, Lorenzo ; Badenes, María Luisa ; Ríos, Gabino</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4277-ec69cdae83fc81a88c1cc8fee643639d59c60d3f4440e77ac2f52c411e4f55973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>13/106</topic><topic>38</topic><topic>38/109</topic><topic>38/111</topic><topic>38/77</topic><topic>631/449/2653</topic><topic>631/449/2661/2665</topic><topic>631/449/447</topic><topic>Cell Proliferation</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Hot Temperature</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Osmotic Pressure</topic><topic>Plant Cells - physiology</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified</topic><topic>Polyubiquitin - metabolism</topic><topic>Protein Interaction Mapping</topic><topic>Prunus persica - genetics</topic><topic>Prunus persica - physiology</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Two-Hybrid System Techniques</topic><topic>Water - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lloret, Alba</creatorcontrib><creatorcontrib>Conejero, Ana</creatorcontrib><creatorcontrib>Leida, Carmen</creatorcontrib><creatorcontrib>Petri, César</creatorcontrib><creatorcontrib>Gil-Muñoz, Francisco</creatorcontrib><creatorcontrib>Burgos, Lorenzo</creatorcontrib><creatorcontrib>Badenes, María Luisa</creatorcontrib><creatorcontrib>Ríos, Gabino</creatorcontrib><collection>SpringerOpen</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lloret, Alba</au><au>Conejero, Ana</au><au>Leida, Carmen</au><au>Petri, César</au><au>Gil-Muñoz, Francisco</au><au>Burgos, Lorenzo</au><au>Badenes, María Luisa</au><au>Ríos, Gabino</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-03-23</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>332</spage><epage>332</epage><pages>332-332</pages><artnum>332</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>We have identified a gene (
PpSAP1
) of
Prunus persica
coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants.
PpSAP1
was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release.
PpSAP1
expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of
PpSAP1
in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as
GROWTH-REGULATING FACTOR
(
GRF
)1-like,
TONOPLAST INTRINSIC PROTEIN
(
TIP
)-like, and
TARGET OF RAPAMYCIN
(
TOR
)-like. Especially, the inverse expression pattern of
PpSAP1
and
TOR
-like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28336950</pmid><doi>10.1038/s41598-017-00471-7</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Scientific reports, 2017-03, Vol.7 (1), p.332-332, Article 332 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_4f63c4695a7742aa93e46064b15a088c |
| source | PMC (PubMed Central); Full-Text Journals in Chemistry (Open access); Publicly Available Content (ProQuest); Springer Nature - nature.com Journals - Fully Open Access |
| subjects | 13/106 38 38/109 38/111 38/77 631/449/2653 631/449/2661/2665 631/449/447 Cell Proliferation Gene Expression Gene Expression Regulation, Plant Hot Temperature Humanities and Social Sciences multidisciplinary Osmotic Pressure Plant Cells - physiology Plant Proteins - metabolism Plants, Genetically Modified Polyubiquitin - metabolism Protein Interaction Mapping Prunus persica - genetics Prunus persica - physiology Science Science (multidisciplinary) Two-Hybrid System Techniques Water - metabolism |
| title | Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus |
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