UV Effect on Human Anterior Lens Capsule Macro-Molecular Composition Studied by Synchrotron-Based FTIR Micro-Spectroscopy

Ultraviolet (UV) irradiation is an important risk factor in cataractogenesis. Lens epithelial cells (LECs), which are a highly metabolically active part of the lens, play an important role in UV-induced cataractogenesis. The purpose of this study was to characterize cell compounds such as nucleic ac...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-05, Vol.22 (10), p.5249
Main Authors: Lumi, Xhevat, Dučić, Tanja, Kreuzer, Martin, Hawlina, Marko, Andjelic, Sofija
Format: Article
Language:English
Subjects:
Acids
Apoptosis
Banded structure
Carbohydrates
Cataracts
Chemical composition
Epithelial cells
Epithelium
Fourier transforms
FTIR
Infrared spectroscopy
Irradiation
lens capsule
lens epithelial cells
Light sources
Lipid peroxidation
Lipids
Macromolecules
Microscopy
Nucleic acids
Oxidative stress
Protein structure
Proteins
Risk analysis
Risk factors
Spectrum analysis
synchrotron light
Synchrotron radiation
Synchrotrons
Ultraviolet radiation
UV irradiation
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Summary:Ultraviolet (UV) irradiation is an important risk factor in cataractogenesis. Lens epithelial cells (LECs), which are a highly metabolically active part of the lens, play an important role in UV-induced cataractogenesis. The purpose of this study was to characterize cell compounds such as nucleic acids, proteins, and lipids in human UV C-irradiated anterior lens capsules (LCs) with LECs, as well as to compare them with the control, non-irradiated LCs of patients without cataract, by using synchrotron radiation-based Fourier transform infrared (SR-FTIR) micro-spectroscopy. In order to understand the effect of the UV C on the LC bio-macromolecules in a context of cataractogenesis, we used the SR-FTIR micro-spectroscopy setup installed on the beamline MIRAS at the Spanish synchrotron light source ALBA, where measurements were set to achieve a single-cell resolution with high spectral stability and high photon flux. UV C irradiation of LCs resulted in a significant effect on protein conformation with protein formation of intramolecular parallel β-sheet structure, lower phosphate and carboxyl bands in fatty acids and amino acids, and oxidative stress markers with significant increase of lipid peroxidation and diminishment of the asymmetric CH3 band.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22105249