Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae)

The karyotype of Pseudapanteles dignus (Muesebeck, 1938), an important parasitoid of a serious tomato pest Phthorimaea (= Tuta ) absoluta Meyrick, 1917 (Lepidoptera, Gelechiidae), in the Neotropics and adjacent regions, was studied for the first time using morphometric analysis and several technique...

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Bibliographic Details
Published in:Comparative cytogenetics 2024-11, Vol.18, p.199-211
Main Authors: Gokhman, Vladimir E., Luna, María Gabriela, Vallina, Consuelo, Bressa, María José
Format: Article
Language:English
Subjects:
Braconidae
chromosome banding
chromosomes
evolution
fish
fluorescence
fluorescent dyes
haploidy
hybridization
karyotyping
morphometry
Neotropics
parasitoids
Phthorimaea
subfamily
tomatoes
Tuta
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Summary:The karyotype of Pseudapanteles dignus (Muesebeck, 1938), an important parasitoid of a serious tomato pest Phthorimaea (= Tuta ) absoluta Meyrick, 1917 (Lepidoptera, Gelechiidae), in the Neotropics and adjacent regions, was studied for the first time using morphometric analysis and several techniques of differential chromosome staining, i.e., C-banding and staining with base-specific fluorochromes, together with fluorescence in situ hybridization (FISH) with an 18S rDNA probe. We found n = 7 and 2n = 14 in P. dignus , with seven metacentric chromosomes of similar size in the haploid set. C-banding revealed various C-positive bands, either centromeric or interstitial, on most chromosomes. Both AT-specific and GC-specific fluorochromes, 4’6-diamidino-2-phenylindole (DAPI) and chromomycin A 3 (CMA 3 ) respectively, showed uniform staining of chromosomes. FISH visualized a single subterminal rDNA site on a medium-sized metacentric. A brief review of known chromosome sets of the subfamily Microgastrinae (Braconidae) is given; certain features of karyotype evolution of this group are discussed.
ISSN:1993-0771
1993-078X
1993-078X
DOI:10.3897/compcytogen.18.133534