Combining Transcriptome and Whole Genome Re-Sequencing to Screen Disease Resistance Genes for Wheat Dwarf Bunt

Wheat dwarf bunt is a damaging disease caused by Kühn (TCK). Once the disease infects wheat, it is difficult to control and will significantly reduce wheat output and quality. RNA sequencing and whole genome re-sequencing were used to search for potential TCK resistance genes in Yili 053 (sensitive...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-12, Vol.24 (24), p.17356
Main Authors: Jia, Yufeng, Shen, Tong, Wen, Zhiwei, Chen, Jing, Liu, Qi
Format: Article
Language:English
Subjects:
Basidiomycota - genetics
Chromosomes
Disease Resistance - genetics
disease resistance gene
Gene expression
Genomes
Infections
Mutation
Pathogens
Plant diseases
Plant Diseases - genetics
Tilletia controversa Kühn
Transcriptome
Triticum - genetics
Wheat
Wheat dwarf bunt
whole genome re-sequencing
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Summary:Wheat dwarf bunt is a damaging disease caused by Kühn (TCK). Once the disease infects wheat, it is difficult to control and will significantly reduce wheat output and quality. RNA sequencing and whole genome re-sequencing were used to search for potential TCK resistance genes in Yili 053 (sensitive variety) and Zhongmai 175 (moderately resistant variety) in the mid-filling, late-filling, and maturity stages. The transcriptomic analysis revealed 11 potential disease resistance genes. An association analysis of the findings from re-sequencing found nine genes with single nucleotide polymorphism mutations. The Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that three up-regulated genes were involved in the synthesis of benzoxazinone and tryptophan metabolism. Additionally, quantitative real-time polymerase chain reaction confirmed the RNA sequencing results. The results revealed novel TCK resistance genes and provide a theoretical basis for researching the function of resistance genes and molecular breeding.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242417356