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Blood-Based Detection of Colorectal Cancer Using Cancer-Specific DNA Methylation Markers

Cancer tissues have characteristic DNA methylation profiles compared with their corresponding normal tissues that can be utilized for cancer diagnosis with liquid biopsy. Using a genome-scale DNA methylation approach, we sought to identify a panel of DNA methylation markers specific for cell-free DN...

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Bibliographic Details
Published in:Diagnostics (Basel) 2020-12, Vol.11 (1), p.51
Main Authors: Cho, Nam-Yun, Park, Ji-Won, Wen, Xianyu, Shin, Yun-Joo, Kang, Jun-Kyu, Song, Sang-Hyun, Kim, Hwang-Phill, Kim, Tae-You, Bae, Jeong Mo, Kang, Gyeong Hoon
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Language:English
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Summary:Cancer tissues have characteristic DNA methylation profiles compared with their corresponding normal tissues that can be utilized for cancer diagnosis with liquid biopsy. Using a genome-scale DNA methylation approach, we sought to identify a panel of DNA methylation markers specific for cell-free DNA (cfDNA) from patients with colorectal cancer (CRC). By comparing DNA methylomes between CRC and normal mucosal tissues or blood leukocytes, we identified eight cancer-specific methylated loci ( , , , , , , , and ) and developed a five-marker panel ( , , , , and ) that detected CRC in liquid biopsies with a high sensitivity and specificity with a droplet digital MethyLight assay. In a set of cfDNA samples from CRC patients ( = 117) and healthy volunteers ( = 60), a panel of five markers on the platform of the droplet digital MethyLight assay detected stages I-III and stage IV CRCs with sensitivities of 45.9% and 95.7%, respectively, and a specificity of 95.0%. The number of detected markers was correlated with the cancer stage, perineural invasion, lymphatic emboli, and venous invasion. Our five-marker panel with the droplet digital MethyLight assay showed a high sensitivity and specificity for the detection of CRC with cfDNA samples from patients with metastatic CRC.
ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics11010051