Filamentation Involves Two Overlapping, but Distinct, Programs of Filamentation in the Pathogenic Fungus Candida albicans

Abstract The ability of the human pathogenic fungus Candida albicans to switch between yeast-like and filamentous forms of growth has long been linked to pathogenesis. Numerous environmental conditions, including growth at high temperatures, nutrient limitation, and exposure to serum, can trigger th...

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Bibliographic Details
Published in:G3 : genes - genomes - genetics 2017-11, Vol.7 (11), p.3797-3808
Main Authors: Azadmanesh, Jahaun, Gowen, Austin M, Creger, Paul E, Schafer, Nichole D, Blankenship, Jill R
Format: Article
Language:English
Subjects:
Candida - genetics
Candida - growth & development
Candida albicans
filamentation
Gene Expression Regulation, Developmental
Gene Expression Regulation, Fungal
Hyphae - genetics
Hyphae - growth & development
hyphal growth
in vitro model comparisons
Investigations
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Summary:Abstract The ability of the human pathogenic fungus Candida albicans to switch between yeast-like and filamentous forms of growth has long been linked to pathogenesis. Numerous environmental conditions, including growth at high temperatures, nutrient limitation, and exposure to serum, can trigger this morphological switch and are frequently used in in vitro models to identify genes with roles in filamentation. Previous work has suggested that differences exist between the various in vitro models both in the genetic requirements for filamentation and transcriptional responses to distinct filamentation-inducing media, but these differences had not been analyzed in detail. We compared 10 in vitro models for filamentation and found broad genetic and transcriptomic differences between model systems. The comparative analysis enabled the discovery of novel media-independent genetic requirements for filamentation as well as a core filamentation transcriptional profile. Our data also suggest that the physical environment drives distinct programs of filamentation in C. albicans, which has significant implications for filamentation in vivo.
ISSN:2160-1836
2160-1836
DOI:10.1534/g3.117.300224