A simple method for squeezing juice from rice stems and its use in the high-throughput analysis of sugar content in rice stems

Sugar content in rice (Oryza sativa L.) stem is an agronomically important trait for rice used in straw silage or whole-crop silage. However, the mechanisms underlying sugar accumulation in rice stems remain unclear, mainly due to the time-consuming method for measuring sugar content. Here, we estab...

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Bibliographic Details
Published in:Plant production science 2016-04, Vol.19 (2), p.309-314
Main Authors: Okamura, Masaki, Hashida, Yoichi, Hirose, Tatsuro, Ohsugi, Ryu, Aoki, Naohiro
Format: Article
Language:English
Subjects:
Agronomy
Brix
Brix value
Compressing
culm
forage rice
Juices
Oryza sativa
Oryza sativa L
Quantitative trait loci
Rice
Silage
Sorghum
Stems
Straw
sucrose
Sugar
Sugarcane
whole-crop silage (WCS)
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Summary:Sugar content in rice (Oryza sativa L.) stem is an agronomically important trait for rice used in straw silage or whole-crop silage. However, the mechanisms underlying sugar accumulation in rice stems remain unclear, mainly due to the time-consuming method for measuring sugar content. Here, we established a simple method for squeezing stem juice from rice plants, similar to that used during breeding selection in sugarcane or sorghum. The Brix value of the stem juice, which can easily be measured using a portable refractometer, significantly correlated with the soluble sugar contents in the stem juice and tended to correlate with those in stem tissues. This indicates that the Brix value of the stem juice can be used for estimating the sugar content in rice stems. This simple estimation method will be a useful tool for high-throughput analysis of sugar content in rice stems during mutant screening, QTL analysis, and breeding selection.
ISSN:1343-943X
1349-1008
DOI:10.1080/1343943X.2015.1128099