Biosynthesis, characterization, and investigation of cytotoxic activities of selenium nanoparticles utilizing Limosilactobacillus fermentum

The biosynthesis of selenium nanoparticles (SeNPs) is one of the methods used alongside other conventional methods for SeNP synthesis. In this research, we used the cell-free culture (CFC) of for SeNP synthesis. We investigated the biosynthesis of SeNPs under various levels of temperature, pH, and S...

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Bibliographic Details
Published in:Green processing and synthesis 2024-08, Vol.13 (1), p.893-11
Main Authors: Aminisough, Seyed Amirhamzeh, Daneshjou, Sara, Khajeh, Khosro
Format: Article
Language:English
Subjects:
Biocompatibility
Biosynthesis
Cell culture
characterization
cytotoxic
Cytotoxicity
Fourier transforms
Free radicals
Infrared analysis
Infrared spectroscopy
Light scattering
Limosilactobacillus fermentum
Nanoparticles
Photon correlation spectroscopy
Polydispersity
Scavenging
Selenium
selenium nanoparticle
Sodium selenite
Spectrum analysis
Toxicity
X-ray diffraction
Zeta potential
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Summary:The biosynthesis of selenium nanoparticles (SeNPs) is one of the methods used alongside other conventional methods for SeNP synthesis. In this research, we used the cell-free culture (CFC) of for SeNP synthesis. We investigated the biosynthesis of SeNPs under various levels of temperature, pH, and Se concentration and characterized the biosynthesized SeNPs using FE-SEM, energy-dispersive X-ray, Fourier transform infrared spectroscopy, X-ray diffraction, ultraviolet–visible spectroscopy (UV–Vis), and dynamic light scattering–zeta potential analyses to find nanoparticles with desirable properties. Also, the cellular toxicity of SeNPs against the MCF-7 cell line was analyzed. The scavenging activity of free radicals in CFC before and after SeNP synthesis was examined using the DPPH method. The selected SeNP has an average hydrodynamic radius of 92.52 nm and a polydispersity index of 0.134. This nanoparticle also has a mostly spherical shape, amorphous nature, and zeta potential of −32.2 mV. The toxicity of nanoparticles for MCF-7 was much lower than sodium selenite salt. It was also confirmed that during nanoparticle synthesis, the reducing ability of CFC significantly decreases. This research aimed to design a safe, cheap, and eco-friendly protocol for the biosynthesis of SeNPs using the CFC of . As a result, SeNPs possess great potential for further exploration in the realm of biomedicine.
ISSN:2191-9542
2191-9550
DOI:10.1515/gps-2024-0030