Analgesic effect of dl-THP on inflammatory pain mediated by suppressing spinal TRPV1 and P2X3 receptors in rats

Tetrahydropalmatine (dl-THP) demonstrates an analgesic effect in animal models of neuropathic and inflammatory pain, however, the underlying mechanisms of its pharmacological action within the spinal cord remains unclear. Both P2X3 receptor and TRPV1 are associated with the development and progressi...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in bioscience (Landmark. Print) 2021-04, Vol.26 (5), p.1-10
Main Authors: Wang, Yan, Wang, Rui-Rui, Sun, Wei, Lou, Chao, Yang, Fan, He, Ting, Wang, Xiao-Liang, Cao, Fa-Le, Chen, Jun
Format: Article
Language:English
Subjects:
Analgesics - pharmacology
Animals
Bee Venoms
Bees
Berberine Alkaloids
dl-thp
Hypersensitivity
Inflammation - drug therapy
inflammatory pain
Male
Motor Skills
Neurons - metabolism
Nociception
p2x3 receptor
Pain - drug therapy
Pain Management - methods
Physical Conditioning, Animal
Rats
Rats, Sprague-Dawley
Receptors, Purinergic P2X3 - metabolism
Spinal Cord - metabolism
Stress, Mechanical
TRPV Cation Channels - metabolism
trpv1
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tetrahydropalmatine (dl-THP) demonstrates an analgesic effect in animal models of neuropathic and inflammatory pain, however, the underlying mechanisms of its pharmacological action within the spinal cord remains unclear. Both P2X3 receptor and TRPV1 are associated with the development and progression of such neuropathic and inflammatory pain. Here, we found that both pre-treatment and post-treatment with dl-THP could attenuate Bee Venom (BV)-induced persistent spontaneous pain-related behaviors in rats. Further, the dl-THP also exerted both preventive and therapeutic analgesic effects in BV-induced primary thermal and mechanical pain hypersensitivity as well as in mirror-image thermal pain hypersensitivity. The Rota-Rod treadmill test revealed that the dl-THP administration did not alter the rats' motor coordinating performance. The TRPV1 and P2X3 receptor proteins increased markedly in the spinal cord of the rats following s.c. BV injection, which was significantly suppressed by dl-THP. These results suggest that dl-THP exerts a robust antihyperalgesia effect through down-regulation of P2X3 receptors and TRPV1 in inflammatory pain, providing a scientific basis for the translation of dl-THP treatment in clinics.
ISSN:2768-6701
2768-6698
DOI:10.52586/4919