Aspen micropropagation: use for phytoremediation of soils

Selected elite Populus tremula and Populus tremula x Populus tremuloides mature trees growing in polluted areas were used for micropropagation. After sterilization of dormant buds, shoot tips were extirpated and cultured on nutrient media. MS medium with BAP (1.0 mg/L) and IBA (0.1 mg/L) showed to b...

Full description

Saved in:
Bibliographic Details
Published in:Journal of forest science (Praha) 2006-03, Vol.52 (3), p.101-107
Main Authors: Mala, J.(Vyzkumny Ustav Lesniho Hospodarstvi a Myslivosti, Jiloviste-Strnady (Czech Republic)) E-mail:mala@vulhm.cz, Machova, P.(Vyzkumny Ustav Lesniho Hospodarstvi a Myslivosti, Jiloviste-Strnady (Czech Republic)), Cvrckova, H.(Vyzkumny Ustav Lesniho Hospodarstvi a Myslivosti, Jiloviste-Strnady (Czech Republic)), Cizkova, L.(Vyzkumny Ustav Lesniho Hospodarstvi a Myslivosti, Jiloviste-Strnady (Czech Republic))
Format: Article
Language:English
Subjects:
AGROBACTERIUM TUMEFACIENS
aspen
BIODECONTAMINACION
BIOREMEDIATION
BROTES
CLONE
CLONES
CULTIVO IN VITRO
CULTURE IN VITRO
CULTURE MEDIA
EXPLANT
EXPLANTES
EXPLANTS
GENE
GENE TRANSFER
GENES
HIBRIDOS
HYBRIDE
HYBRIDS
IN VITRO CULTURE
IN VITRO REGENERATION
MEDIO DE CULTIVO
MICROPROPAGACION
MICROPROPAGATION
MILIEU DE CULTURE
MULTIPLICATION DES PLANTES
phytoremediation
PLANT PROPAGATION
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
POPULUS TREMULA
POPULUS TREMULOIDES
POUSSE
PROPAGACION DE PLANTAS
REGENERACION IN VITRO
REGENERATION IN VITRO
SHOOTS
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
TRANSGENIC PLANTS
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Selected elite Populus tremula and Populus tremula x Populus tremuloides mature trees growing in polluted areas were used for micropropagation. After sterilization of dormant buds, shoot tips were extirpated and cultured on nutrient media. MS medium with BAP (1.0 mg/L) and IBA (0.1 mg/L) showed to be suitable for organogenesis induction, whereas MS medium with BAP (0.2 mg/L) and glutamine (100 mg/L) was used for multiplication. A high number of adventitious shoots (20-30) was produced per multi-apex culture. Twenty-five clones were established in the gene bank. Four gene constructs of Agrobacterium tumefaciens comprising different promoters were used for transformations of aspen hybrid stem segments (bearing two genes, NPTII coding resistance to kanamycin, and GUS coding glucuronidase synthesis). The highest GUS activity was estimated in transformants with construct 148 controlled by promoter CAMV 35S.
ISSN:1212-4834
1805-935X
DOI:10.17221/4491-jfs