Post Natal Microbial and Metabolite Transmission: The Path from Mother to Infant

• Published in: Nutrients 2024-07, Vol.16 (13), p.1990
• Main Authors: Vélez-Ixta, Juan Manuel, Juárez-Castelán, Carmen Josefina, Ramírez-Sánchez, Daniela, Lázaro-Pérez, Noemí Del Socorro, Castro-Arellano, José Javier, Romero-Maldonado, Silvia, Rico-Arzate, Enrique, Hoyo-Vadillo, Carlos, Salgado-Mancilla, Marisol, Gómez-Cruz, Carlos Yamel, Krishnakumar, Aparna, Piña-Escobedo, Alberto, Benitez-Guerrero, Tizziani, Pizano-Zárate, María Luisa, Cruz-Narváez, Yair, García-Mena, Jaime
• Format: Article
• Language: English
• Subjects: 16S RNA gene; Adult; Analysis; Babies; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - metabolism; Breast Feeding; Breastfeeding & lactation; Cesarean section; Comparative analysis; Composition; Development; entero–mammary pathway; Fatty acids; Feces; Feces - microbiology; Female; Gastrointestinal Microbiome - physiology; Humans; Infant; Infant, Newborn; Infants; Influence; Male; mass spectrometry; Maternal-fetal exchange; Metabolism; Metabolites; Metabolome; Metabolomics; microbiome; Microbiota; Microbiota (Symbiotic organisms); Milk; Milk, Human - chemistry; Milk, Human - microbiology; Mothers; Newborn babies; Obesity; Pregnancy; Questionnaires; Vagina; Womens health
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu16131990

The entero-mammary pathway is a specialized route that selectively translocates bacteria to the newborn's gut, playing a crucial role in neonatal development. Previous studies report shared bacterial and archaeal taxa between human milk and neonatal intestine. However, the functional implications for neonatal development are not fully understood due to limited evidence. This study aimed to identify and characterize the microbiota and metabolome of human milk, mother, and infant stool samples using high-throughput DNA sequencing and FT-ICR MS methodology at delivery and 4 months post-partum. Twenty-one mothers and twenty-five infants were included in this study. Our results on bacterial composition suggest vertical transmission of bacteria through breastfeeding, with major changes occurring during the first 4 months of life. Metabolite chemical characterization sheds light on the growing complexity of the metabolites. Further data integration and network analysis disclosed the interactions between different bacteria and metabolites in the biological system as well as possible unknown pathways. Our findings suggest a shared bacteriome in breastfed mother-neonate pairs, influenced by maternal lifestyle and delivery conditions, serving as probiotic agents in infants for their healthy development. Also, the presence of food biomarkers in infants suggests their origin from breast milk, implying selective vertical transmission of these features.