Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene

Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. We identified 1 K189T subst...

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Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical 2019-01, Vol.52, p.e20180225-e20180225
Main Authors: Chapadense, Francesca, Machado, Ricardo Luiz Dantas, Ventura, Ana Maria Revorêdo da Silva, Áreas, André, Machado, Renato Beilner, Viana, Giselle Rachid, Zalis, Mariano Gustavo, Castro, Ana Maria de, Cravo, Pedro
Format: Article
Language:English
Subjects:
Artemisinin Resistance
Artemisinins - pharmacology
Drug Resistance - genetics
Genotype
Humans
Kelch 13
Malaria
Malaria, Falciparum - parasitology
Mutation - genetics
Phenotype
Plasmodium falciparum
Plasmodium falciparum - drug effects
Plasmodium falciparum - genetics
Protozoan Proteins - genetics
TROPICAL MEDICINE
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Summary:Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples. Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.
ISSN:0037-8682
1678-9849
1678-9849
DOI:10.1590/0037-8682-0225-2018