Inhibitory Effects of Erythrosine/Curcumin Derivatives/Nano-Titanium Dioxide-Mediated Photodynamic Therapy on Candida albicans

• Published in: Molecules (Basel, Switzerland) Switzerland), 2021-04, Vol.26 (9), p.2405
• Main Authors: Kanpittaya, Kasama, Teerakapong, Aroon, Morales, Noppawan Phumala, Hormdee, Doosadee, Priprem, Aroonsri, Weera-Archakul, Wilawan, Damrongrungruang, Teerasak
• Format: Article
• Language: English
• Subjects: Antifungal Agents - chemistry; Antifungal Agents - pharmacology; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Binding sites; biofilm; Biofilms; Biofilms - drug effects; Candida albicans; Candida albicans - drug effects; Colonies; Composite materials; Curcumin; Curcumin - chemistry; Curcumin - pharmacology; curcumin derivatives; Disinfection & disinfectants; Drug resistance; Electron paramagnetic resonance; Electron spin resonance; Electron Spin Resonance Spectroscopy; erythrosine; Erythrosine - chemistry; Fibroblasts - metabolism; Free radicals; Fungicides; Gingiva - cytology; Hydrogen bonds; Hydroxyl radicals; Infections; Irradiation; Microorganisms; Nystatin; Photochemotherapy; Photodynamic therapy; Photosensitizing Agents - chemistry; Photosensitizing Agents - pharmacology; Reactive Oxygen Species - metabolism; Titanium; Titanium - chemistry; Titanium dioxide; Toxicity; Toxicity testing
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26092405

This study focuses on the role of photosensitizers in photodynamic therapy. The photosensitizers were prepared in combinations of 110/220 µM erythrosine and/or 10/20 µM demethoxy/bisdemethoxy curcumin with/without 10% ( ) nano-titanium dioxide. Irradiation was performed with a dental blue light in the 395-480 nm wavelength range, with a power density of 3200 mW/cm and yield of 72 J/cm . The production of ROS and hydroxyl radical was investigated using an electron paramagnetic resonance spectrometer for each individual photosensitizer or in photosensitizer combinations. Subsequently, a PrestoBlue toxicity test of the gingival fibroblast cells was performed at 6 and 24 h on the eight highest ROS-generating photosensitizers containing curcumin derivatives and erythrosine 220 µM. Finally, the antifungal ability of 22 test photosensitizers, (ATCC 10231), were cultured in biofilm form at 37 °C for 48 h, then the colonies were counted in colony-forming units (CFU/mL) via the drop plate technique, and then the log reduction was calculated. The results showed that at 48 h the test photosensitizers could simultaneously produce both ROS types. All test photosensitizers demonstrated no toxicity on the fibroblast cells. In total, 18 test photosensitizers were able to inhibit similarly to nystatin. Conclusively, 20 µM bisdemethoxy curcumin + 220 µM erythrosine + 10% ( ) nano-titanium dioxide exerted the highest inhibitory effect on .