In Vitro Antioxidant and Antiaging Activities of Collagen and Its Hydrolysate from Mackerel Scad Skin (Decapterus macarellus)

• Published in: Marine drugs 2022-08, Vol.20 (8), p.516
• Main Authors: Herawati, Elisa, Akhsanitaqwim, Yochidamai, Agnesia, Pipin, Listyawati, Shanti, Pangastuti, Artini, Ratriyanto, Adi
• Format: Article
• Language: English
• Subjects: Acetic acid; Aging; antiaging; antioxidant; Antioxidants; Collagen; Collagen (type I); Collagen (type II); Collagenase; Decapterus macarellus; Enzymes; Fish; Fish skin; Fourier analysis; Fourier transforms; Gel electrophoresis; Hydrolysates; hydrolyzed collagen; Infrared analysis; Levodopa; Low income groups; Mackerel; mackerel scad skin; Marine fishes; Molecular weight; Morphology; Pepsin; Peptides; pH effects; Proteins; Scanning electron microscopy; Sodium lauryl sulfate; Spectrum analysis; Substrates; Tuna
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md20080516

The skin of mackerel scad fish (Decapterus macarellus) is a new source for pepsin-soluble collagen and its hydrolysate, both of which have never been explored. This study aims to characterize and determine the in vitro antioxidant, antiglycation, and antityrosinase activity of pepsin-soluble collagen (PSC) and hydrolyzed collagen (HC) from mackerel scad skin. PSC was extracted using 0.5 M acetic acid containing 0.1% pepsin for 48 h at 4 °C. The obtained PSC was then hydrolyzed with collagenase type II (6250 U/g) to produce HC. The PSC yield obtained was 6.39 ± 0.97%, with a pH of 6.76 ± 0.18, while the HC yield was 96% from PSC. SDS-PAGE and Fourier Transform Infrared (FTIR) analysis showed the typical features of type I collagen. HC demonstrated high solubility (66.75–100%) throughout the entire pH range (1–10). The PSC and HC from mackerel scad skin showed antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), with IC50 values of 148.55 ± 3.14 ppm and 34.966 ± 0.518 ppm, respectively. In the antiglycation test, PSC had an IC50 value of 239.29 ± 15.67 ppm, while HC had an IC50 of 68.43 ± 0.44 ppm. PSC also exhibited antityrosinase activity, with IC50 values of 234.66 ± 0.185 ppm (on the L-DOPA substrate), while HC had an IC50 value of 79.35 ± 0.5 ppm. Taken together, these results suggest that the skin of mackerel scad fish has potential antiaging properties and can be further developed for pharmaceutical and cosmetic purposes.