Bioluminescent imaging in induced mouse models of endometriosis reveals differences in four model variations

Our understanding of the aetiology and pathophysiology of endometriosis remains limited. Disease modelling in the field is problematic as many versions of induced mouse models of endometriosis exist. We integrated bioluminescent imaging of 'lesions' generated using luciferase-expressing do...

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Bibliographic Details
Published in:Disease models & mechanisms 2021-08, Vol.14 (8)
Main Authors: Dorning, Ashley, Dhami, Priya, Panir, Kavita, Hogg, Chloe, Park, Emma, Ferguson, Gregory D, Hargrove, Diane, Karras, James, Horne, Andrew W, Greaves, Erin
Format: Article
Language:English
Subjects:
Abdomen
Animals
Disease Models, Animal
Endometriosis
Endometriosis - diagnostic imaging
Endometriosis - pathology
Endometrium
Female
gnrh antagonist
Histology
Hormone Antagonists - pharmacology
Humans
lesion
Menstruation
Mice
Mouse as a Disease Model
Ovaries
pain
Rodents
Transplants & implants
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Summary:Our understanding of the aetiology and pathophysiology of endometriosis remains limited. Disease modelling in the field is problematic as many versions of induced mouse models of endometriosis exist. We integrated bioluminescent imaging of 'lesions' generated using luciferase-expressing donor mice. We compared longitudinal bioluminescence and histology of lesions, sensory behaviour of mice with induced endometriosis and the impact of the gonadotropin-releasing hormone antagonist Cetrorelix on lesion regression and sensory behaviour. Four models of endometriosis were tested. We found that the nature of the donor uterine material was a key determinant of how chronic the lesions were, as well as their cellular composition. The severity of pain-like behaviour also varied across models. Although Cetrorelix significantly reduced lesion bioluminescence in all models, it had varying impacts on pain-like behaviour. Collectively, our results demonstrate key differences in the progression of the 'disease' across different mouse models of endometriosis. We propose that validation and testing in multiple models, each of which may be representative of the different subtypes/heterogeneity observed in women, should become a standard approach to discovery science in the field of endometriosis.
ISSN:1754-8403
1754-8411
1754-8411
DOI:10.1242/dmm.049070