Effect of Doublesynch and Estradoublesynch protocols on estrus induction, conception rate, plasma progesterone, protein, and cholesterol profile in anestrus Gir heifers

• Published in: Veterinary World 2018-04, Vol.11 (4), p.542-548
• Main Authors: Chaudhary, N J, Patel, D M, Dhami, A J, Vala, K B, Hadiya, K K, Patel, J A
• Format: Article
• Language: English
• Subjects: Anestrus; Animal behavior; Animals; Artificial insemination; Beef cattle; Biotin; Blood proteins; Cattle; Cholesterol; conception rate; Estrus; estrus synchronization; Gir heifers; Injection; Ivermectin; Minerals; Organic phosphorus; Phosphorus; Progesterone; Prostaglandins; Proteins; pubertal anestrus; Sex hormones; Vitamin A; Vitamins
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2018.542-548

This study aimed to evaluate the efficacy of Doublesynch and Estradoublesynch protocols on estrus induction, conception rates, plasma progesterone, protein, and cholesterol profile in anestrus Gir heifers. In this study, 50 pubertal anestrus Gir heifers were selected from the field and farm conditions. The heifers were dewormed (injection ivermectin, 100 mg, s/c) and supplemented with minerals and vitamins (injection organic phosphorus 800 mg and injection Vitamin AD E and Biotin 10 ml i/m) and multi-mineral bolus at 1 bolus daily for 7 days. The heifers were randomly divided into three groups: Doublesynch (n=20), Estradoublesynch (n=20), and control (n=10). The animals were monitored for estrus response, estrus interval, behavioral signs, and conception rates after induced/first, second, and third cycle post-treatment. Blood samples were obtained on day 0, day 9, day 12, and on day 12 post-artificial insemination (AI) for determination of plasma progesterone, protein, and cholesterol profile. The estrus response rate between Doublesynch and Estradoublesynch protocols was similar between treated heifers (85% and 95%). The interval from the second prostaglandin F2α (PGF α) injection to estrus induction did not differ between the groups (63.87±4.19 vs. 58.27±3.83 h). The conception rates following induced estrus (20% vs. 30%), at the second cycle (23.07% vs. 16.66%), at the third cycle (22.22% vs. 30.00%), and the overall conception rate (45% and 55%) within 27.89±5.75 and 26.45±5.48 days were the same across the treatment groups. The mean plasma progesterone concentrations were significantly (p