BrightMice: a low-cost do-it-yourself instrument, designed for in vivo fluorescence mouse imaging

In vivo fluorescent imaging represents a potent means for real-time probe quantification, facilitating insights into disease pathophysiology and therapeutic responses. Nonetheless, accurate signal quantification remains challenging due to inherent factors like light scattering and tissue absorption....

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Bibliographic Details
Published in:Scientific reports 2024-09, Vol.14 (1), p.22685-13, Article 22685
Main Authors: Boitet, Maylis, Achek, Asma, Bouchenaki, Kelian, Grailhe, Regis
Format: Article
Language:English
Subjects:
3-D printers
631/1647/2017/1958
631/1647/245/2225
Adaptability
Albinism
Animals
Comparative analysis
Digital cameras
Efficiency
Fluorescence
Fluorescent Dyes - chemistry
Fluorophores
Humanities and Social Sciences
Laboratories
Light emitting diodes
Light scattering
Mice
multidisciplinary
Optical Imaging - methods
Pathophysiology
Printing, Three-Dimensional
Proteins
Science
Science (multidisciplinary)
Tomography
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Summary:In vivo fluorescent imaging represents a potent means for real-time probe quantification, facilitating insights into disease pathophysiology and therapeutic responses. Nonetheless, accurate signal quantification remains challenging due to inherent factors like light scattering and tissue absorption. Existing imaging systems, though sophisticated, often entail high costs and are typically restricted to well-funded laboratory settings. This study introduces BrightMice, an innovative in vivo fluorescent imaging system that harnesses 3D printing and consumer-grade digital cameras. Tailored for various fluorophores such as EYFP and E2-crimson, the system showcases both adaptability and effectiveness in detecting in vivo fluorescent signals in several reporter mouse strains. Comparative analyses against commercial instruments confirm BrightMice’s sensitivity and underscore its potential to democratize in vivo fluorescence imaging. By providing a cost-effective and accessible solution, BrightMice stands to benefit diverse research environments.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-73130-3