Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)

The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous...

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Bibliographic Details
Published in:Genetics and molecular biology 2019-01, Vol.42 (1), p.87-94
Main Authors: Bombonato, Juliana Rodrigues, Bonatelli, Isabel Aparecida Silva, Silva, Gislaine Angélica Rodrigues, Moraes, Evandro Marsola, Zappi, Daniela Cristina, Taylor, Nigel P, Franco, Fernando Faria
Format: Article
Language:English
Subjects:
Amplification
BIOCHEMISTRY & MOLECULAR BIOLOGY
Cactaceae
Cacti
Case studies
Cereus
Cluster analysis
Clustering
cross-genera
Genetic diversity
Genetics
GENETICS & HEREDITY
Markers
Plant Genetics
Population genetics
Population studies
Primers
Simple sequence repeats
SSR markers
Transferability
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Summary:The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus.
ISSN:1415-4757
1678-4685
1678-4685
DOI:10.1590/1678-4685-GMB-2017-0293