Effect of erbium yttrium aluminium garnet laser dentin conditioning on dental pulp stem cells viability

• Published in: Heliyon 2024-03, Vol.10 (5), p.e26954-e26954, Article e26954
• Main Authors: Jafari, Aryan, Vatanpour, Mehdi, Barikrow, Nooshin, Razavi, Pouyan, Tour Savadkouhi, Sohrab
• Format: Article
• Language: English
• Subjects: Dentin; EDTA; Erbium YAG laser; Mesenchymal stem cells; Regenerative endodontics
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2024.e26954

This study aims to investigate the effect of dentin conditioning by subablative Er:YAG (erbium-doped yttrium aluminium garnet) laser on dental pulp stem cells (DPSCs) viability. For this in-vitro experimental study, root fragments were longitudinally hemisected after decoronation of single-rooted extracted teeth and preparation of root canals. Prepared samples were randomly assigned to 2 experimental groups (n = 17) as follows; 1) laser conditioning: irradiation with Er:YAG laser beams (2940 nm, 50 mJ per pulse, 20 Hz) 2) Chemical conditioning: 1.5% NaOCl, followed by phosphate-buffered saline (PBS), 17% EDTA, followed by PBS as a final rinse. The samples were ultraviolet-sterilized, and DPSCs were seeded on the samples. MTT assay was performed after 1, 4 and 7 days of incubation to assess the cell viability (n = 5/group per day). Also, after 7 days, two samples of each group underwent SEM (scanning electron microscope) analysis. Statistical analysis was done using independent t-test, one-way ANOVA and two-way ANOVA at a significance level of 0.05. Laser irradiated samples exhibited significantly higher cell viability of DPSCs on days 4 (p