Lactobacillus paracasei treatment modulates mRNA expression in macrophages

Macrophage metabolic pathways show changes in response to various external stimuli. Especially, increased lipopolysaccharide, an important bacterial component and Toll-like receptor 4 agonist, can induce activity in various macrophage metabolic pathways, including energy production and biosynthesis,...

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Bibliographic Details
Published in:Biochemistry and biophysics reports 2020-09, Vol.23, p.100788, Article 100788
Main Authors: Han, Dae Hee, Kim, Woon-Ki, Park, SungJun, Jang, You Jin, Ko, GwangPyo
Format: Article
Language:English
Subjects:
Glycolysis
Immunomodulation
Lactobacillus paracasei
Macrophage
Macrophage polarization
Probiotics
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Summary:Macrophage metabolic pathways show changes in response to various external stimuli. Especially, increased lipopolysaccharide, an important bacterial component and Toll-like receptor 4 agonist, can induce activity in various macrophage metabolic pathways, including energy production and biosynthesis, as well as high immune responses due to increase in differentiated M1 macrophages. In this study, we confirmed that Lactobacillus paracasei (L. paracasei) KBL382, KBL384 and KBL385, isolated from the feces of healthy Koreans, can modulate various enzymes and membrane transporters related to glycolysis or macrophage polarization including hypoxia-inducible factor 1-alpha (HIF1A), inducible nitric oxide synthase (iNOS) and arginase in stimulated macrophages at the mRNA level, using the in vitro rodent bone‐marrow‐derived macrophage (BMDM) model. All L. paracasei exhibited significant down-regulatory effects on mRNAs for glycolysis-related enzymes, including lactate dehydrogenase A, solute carrier family 2 member 1, and triosephosphate isomerase. Moreover, L. paracasei treatment could lead to significant reductions in HIF1A or iNOS mRNA, and induced arginase mRNA in the BMDM model. Therefore, further extensive studies should be performed to support the application of L. paracasei, such as in probiotics or therapeutics, in controlling abnormal immune responses related to macrophage. •Macrophage metabolic pathways can be altered by the various stimuli.•L. paracasei treatment reduces glycolysis-related mRNA in the BMDM model.•L. paracasei treatment modulates mRNA in association with macrophage polarization.•L. paracasei could be useful to control macrophage-mediated immune responses.
ISSN:2405-5808
2405-5808
DOI:10.1016/j.bbrep.2020.100788