Development and characterization of segment-specific enteroids from the pig small intestine in Matrigel and transwell inserts: insights into susceptibility to porcine epidemic diarrhea Virus

• Published in: Frontiers in immunology 2024-09, Vol.15, p.1451154
• Main Authors: Yen, Lu, Nelli, Rahul K, Twu, Ning-Chieh, Mora-Díaz, Juan Carlos, Castillo, Gino, Sitthicharoenchai, Panchan, Giménez-Lirola, Luis G
• Format: Article
• Language: English
• Subjects: alphacoronavirus; Animals; Cells, Cultured; Collagen - metabolism; Coronavirus Infections - immunology; Coronavirus Infections - veterinary; Coronavirus Infections - virology; Disease Susceptibility; Drug Combinations; Immunology; infection; Intestinal Mucosa - immunology; Intestinal Mucosa - virology; Intestine, Small - immunology; Intestine, Small - virology; Laminin; Matrigel; organoids; Organoids - virology; porcine enteroids; porcine epidemic diarrhea virus; Porcine epidemic diarrhea virus - physiology; Proteoglycans; Swine; Swine Diseases - immunology; Swine Diseases - virology
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2024.1451154

The critical early stages of infection and innate immune responses to porcine epidemic diarrhea virus (PEDV) at the intestinal epithelium remain underexplored due to the limitations of traditional cell culture and animal models. This study aims to establish a porcine enteroid culture model to investigate potential differences in susceptibility to infection across segments of the porcine small intestine (duodenum, jejunum, and ileum). Intestinal crypt cells from nursery pigs were cultured in Matrigel to differentiate into porcine enteroid monolayer cultures (PEMCs). Following characterization, PEMCs were enzymatically dissociated and subcultured on transwell inserts (PETCs) for apical surface exposure and infection studies. Characterization of region-specific PEMCs and PETCs included assessment of morphology, proliferation, viability, and cellular phenotyping via immunohistochemistry/immunocytochemistry and gene expression analysis. Subsequently, PETCs were inoculated with 10 TCID (50% tissue culture infectious dose)/mL of a high pathogenic PEDV non-S INDEL strain and incubated for 24 h. Infection outcomes were assessed by cytopathic effect, PEDV N protein expression (immunofluorescence assay, IFA), and PEDV N-gene detection (quantitative reverse transcription polymerase chain reaction, RT-qPCR). No significant morphological and phenotypical differences were observed among PEMCs and PETCs across intestinal regions, resembling the porcine intestinal epithelium. Although PETCs established from different segments of the small intestine were susceptible to PEDV infection, jejunum-derived PETCs exhibited higher PEDV replication, confirmed by IFA and RT-qPCR. This segment-specific enteroid culture model provides a reliable platform for virological studies, offering a controlled environment that overcomes the limitations of and traditional cell culture methods. Standardizing culture conditions and characterizing the model are essential for advancing enteroid-based infection models.