Superior anti-pulmonary viral potential of Natrialba sp. M6-producing surfactin and C50 carotenoid pigment with unveiling its action modes

• Published in: Virology journal 2023-10, Vol.20 (1), p.1-249, Article 249
• Main Authors: Hegazy, Ghada E, Abu-Serie, Marwa M, Soliman, Nadia A, Teleb, Mohamed, Abdel-Fattah, Yasser R
• Format: Article
• Language: English
• Subjects: Acidification; Acute respiratory distress syndrome; Adenoviruses; Adsorption; Analysis; Antioxidants; Antiviral agents; Capsid protein; Carotenoids; Cell lines; Chymotrypsin; Complications and side effects; Coronaviruses; Cytotoxicity; Diagnosis; Disease transmission; DNA polymerase; DNA-directed DNA polymerase; Drug therapy; Egypt; Endocrine system; Evaluation; Exo-a-sialidase; Genomes; Hemagglutinins; Hepatitis; Herpes simplex; High performance liquid chromatography; Infections; Influenza; Influenza A; Lectins; Life cycles; Mortality; Pandemics; Proteases; Protein binding; Public health; Public health administration; Respiratory distress syndrome; RNA polymerase; Severe acute respiratory syndrome coronavirus 2; Surface active agents; Surfactants; Surfactin; Swine; Viral infections; Viral proteins; Viruses
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/s12985-023-02215-8

Respiratory viruses, particularly adenoviruses (ADV), influenza A virus (e.g., H1N1), and coronaviruses (e.g., HCoV-229E and SARS-CoV-2) pose a global public health problem. Therefore, developing natural wide-spectrum antiviral compounds for disrupting the viral life cycle with antioxidant activity provides an efficient treatment approach. Herein, biosurfactant (Sur) and C50 carotenoid pigment (Pig) of haloalkaliphilic archaeon Natrialba sp. M6 which exhibited potent efficacy against hepatitis and anti-herpes simplex viruses, were investigated against pulmonary viruses. The cytotoxicity of the extracted Sur and Pig was examined on susceptible cell lines for ADV, HIN1, HCoV-229E, and SARS-CoV-2. Their potential against the cytopathic activity of these viruses was detected with investigating the action modes (including, virucidal, anti-adsorption, and anti-replication), unveiling the main mechanisms, and using molecular docking analysis. Radical scavenging activity was determined and HPLC analysis for potent extract (Sur) was performed. All current investigations stated higher anti-pulmonary viruses of Sur than Pig via mainly virucidal and/or anti-replicative modes. Moreover, Sur had stronger ADV's capsid protein binding, ADV's DNA polymerase inhibition, suppressing hemagglutinin and neuraminidase of H1N1, and inhibiting chymotrypsin-like (3CL) protease of SARS-CoV-2, supporting with in-silico analysis, as well as radical scavenging activity than Pig. HPLC analysis of Sur confirmed the predominate presence of surfactin in it. This study declared the promising efficacy of Sur as an efficient pharmacological treatment option for these pulmonary viruses and considered as guide for further in vivo research.