Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84

• Published in: BMC genomics 2017-09, Vol.18 (1), p.701-12, Article 701
• Main Authors: Teunissen, Janneke H M, Crooijmans, Marjolein E, Teunisse, Pepijn P P, van Heusden, G Paul H
• Format: Article
• Language: English
• Subjects: 14-3-3 protein; 14-3-3 proteins; 14-3-3 Proteins - deficiency; 14-3-3 Proteins - genetics; Affinity; Annotations; Biological Transport - genetics; Brewer's yeast; Clonal deletion; Cyclin-Dependent Kinase Inhibitor Proteins - genetics; Cyclin-dependent kinases; Deoxyribonucleic acid; Disruption; DNA; Flow cytometry; Gene Deletion; Gene expression; Gene Expression Regulation, Fungal; Genes; Genetic aspects; Genomes; Genomics; Heterogeneity; Homeostasis; Ion channels; Kinases; Metabolism; Mutants; Observations; PHO regulon; PHO84; Phosphate; Phosphates; Phosphates - metabolism; Physiological aspects; Plasmids; Population genetics; Potassium; Potassium - metabolism; Proteins; Proton-Phosphate Symporters - genetics; Regulators; Saccharomyces cerevisiae; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - genetics; Signal processing; Signal transduction; SPL2; Strains (organisms); Transcription factors; Yeast; Yeasts
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-017-4105-8

Ion homeostasis is an essential property of living organisms. The yeast Saccharomyces cerevisiae is an ideal model organism to investigate ion homeostasis at all levels. In this yeast genes involved in high-affinity phosphate uptake (PHO genes) are strongly induced during both phosphate and potassium starvation, indicating a link between phosphate and potassium homeostasis. However, the signal transduction processes involved are not completely understood. As 14-3-3 proteins are key regulators of signal transduction processes, we investigated the effect of deletion of the 14-3-3 genes BMH1 or BMH2 on gene expression during potassium starvation and focused especially on the expression of genes involved in phosphate uptake. Genome-wide analysis of the effect of disruption of either BMH1 or BMH2 revealed that the mRNA levels of the PHO genes PHO84 and SPL2 are greatly reduced in the mutant strains compared to the levels in wild type strains. This was especially apparent at standard potassium and phosphate concentrations. Furthermore the promoter of these genes is less active after deletion of BMH1. Microscopic and flow cytometric analysis of cells with GFP-tagged SPL2 showed that disruption of BMH1 resulted in two populations of genetically identical cells, cells expressing the protein and the majority of cells with no detectible expression. Heterogeneity was also observed for the expression of GFP under control of the PHO84 promoter. Upon deletion of PHO80 encoding a regulator of the transcription factor Pho4, the effect of the BMH1 deletion on SPL2 and PHO84 promoter was lost, suggesting that the BMH1 deletion mainly influences processes upstream of the Pho4 transcription factor. Our data indicate that that yeast cells can be in either of two states, expressing or not expressing genes required for high-affinity phosphate uptake and that 14-3-3 proteins are involved in the process(es) that establish the activation state of the PHO regulon.