Development of Functionalized Poly(ε-caprolactone)/Hydroxyapatite Scaffolds via Electrospinning 3D for Enhanced Bone Regeneration

• Published in: ACS omega 2024-11, Vol.9 (45), p.45035-45046
• Main Authors: Lima, Maria José da Silva, de Melo, Etelino Feijó, Alves, Kleber G. B., de Sá, Fabrício Bezerra, Alves Júnior, Severino
• Format: Article
• Language: English
• ISSN: 2470-1343; 2470-1343
• DOI: 10.1021/acsomega.4c05264

Functionalized scaffolds based on biodegradable polymers are materials used in bone tissue engineering. This study presents the development of functionalized fibrous scaffolds, fabricated from poly­(ε-caprolactone) (PCL) and hydroxyapatite (HA). To produce this material, a short-distance electrospinning (ES) system was developed by adapting a 3D printer. The morphology and chemical properties of the scaffolds were evaluated using scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. The results confirmed the porous structure and the presence of hydroxyapatite throughout the entire scaffold area. Mechanical tests indicated good elasticity and tensile strength of the scaffolds, favorable for bone regeneration. In vitro tests showed high levels of cell viability. Furthermore, in vivo experiments using a calvarial defect model in rats demonstrated that the PCL/HA scaffold promoted enhanced bone regeneration. Therefore, the PCL/HA scaffold developed through the adapted electrospinning system shows promise for bone repair.