Site-Specific Radioiodination of Oligonucleotides with a Phenolic Element in a Programmable Approach

Radioiodination of oligonucleotides provides an extra modality for nucleic acid-based theranostics with potential applications. Herein, we report the design and synthesis of a phosphoramidite embedded with a phenolic moiety and demonstrate that oligonucleotides can be readily functionalized with phe...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-09, Vol.27 (19), p.6257
Main Authors: Zhao, Haitao, Qin, Yu, Liu, Dunfang, Geng, Xinyao, Wang, Cheng, Ding, Ding, Ding, Xuan, Xia, Qian, Liu, Jianjun, Wang, Ruowen, Tan, Weihong
Format: Article
Language:English
Subjects:
aptamer
Aptamers
Biodistribution
Deoxyribonucleic acid
DNA
DNA biosynthesis
DNA synthesis
Flow cytometry
Gene expression
Incorporation
Iodine
Kinases
Nucleic acids
oligonucleotide
Phenolic compounds
Phenols
phosphoramidite
Precision medicine
Precursors
Proteins
Radioiodination
RNA
solid-phase synthesis
Tomography
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Summary:Radioiodination of oligonucleotides provides an extra modality for nucleic acid-based theranostics with potential applications. Herein, we report the design and synthesis of a phosphoramidite embedded with a phenolic moiety and demonstrate that oligonucleotides can be readily functionalized with phenol as a precursor by general DNA synthesis. It was identified that the introduction of the precursor does not block the specificity of an aptamer, and the radioiodination is applicable to both DNA and RNA oligonucleotides in a site-specific approach with a commercial kit.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27196257