Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1

• Published in: Marine drugs 2022-02, Vol.20 (2), p.116
• Main Authors: Pliego-Cortés, Hugo, Hardouin, Kévin, Bedoux, Gilles, Marty, Christel, Cérantola, Stéphane, Freile-Pelegrín, Yolanda, Robledo, Daniel, Bourgougnon, Nathalie
• Format: Article
• Language: English
• Subjects: Algae; Alzheimer's disease; Animals; Antiviral activity; Antiviral agents; Antiviral Agents - isolation & purification; Antiviral Agents - pharmacology; Chemical composition; Chlorocebus aethiops; Cytotoxicity; Disease transmission; Drug resistance; Enzymes; France; Halymenia floresii; Herpes simplex; Herpes viruses; Herpesvirus 1, Human - drug effects; HIV; Human immunodeficiency virus; Infections; Ion exchange; Life Sciences; Mexico; Molecular Weight; Natural products; Neurodegenerative diseases; NMR; Nuclear magnetic resonance; Polysaccharides; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; red seaweed; Saccharides; Sargassum muticum; Seaweed - chemistry; Seaweeds; Solieria chordalis; Stranding; Sulfates; Toxicity; Ulva sp; Vero Cells; Viral infections; Viruses
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md20020116

virus 1 (HSV-1) remains a prominent health concern widespread all over the world. The increasing genital infections by HSV-1 that might facilitate acquisition and transmission of HIV-1, the cumulative evidence that HSV-1 promotes neurodegenerative disorders, and the emergence of drug resistance signify the need for new antiviral agents. In this study, the in vitro anti-herpetic activity of sulfated polysaccharides (SPs) extracted by enzyme or hot water from seaweeds collected in France and Mexico from stranding events, were evaluated. The anti-herpetic activity evaluation of the semi-refined-polysaccharides (sr-SPs) and different ion exchange purified fractions showed a wide range of antiviral activity. Among them, the sr-SPs from the Rhodophyta showed stronger activity EC 0.68 μg/mL with SI 1470, without cytotoxicity. Further, the antiviral activity of the sr-SPs evaluated at different treatment schemes showed a high EC of 0.38 μg/mL during the viral adsorption assays when the polysaccharide and the virus were added simultaneously, whilst the protection on Vero cell during the post-infection assay was effective up to 1 h. The chemical composition, FTIR and H NMR spectroscopic, and molecular weights of the sr-SPs from were determined and discussed based on the anti-herpetic activity. The potential utilization of seaweed stranding as a source of antiviral compounds is addressed.