Development of bioluminescent Salmonella strains for use in food safety

Salmonella can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperature during preparation, salmonellosis can occur. In this research, we developed biolumin...

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Bibliographic Details
Published in:BMC microbiology 2008-01, Vol.8 (1), p.10-10, Article 10
Main Authors: Karsi, Attila, Howe, Kevin, Kirkpatrick, Tasha B, Wills, Robert, Bailey, R Hartford, Lawrence, Mark L
Format: Article
Language:English
Subjects:
Animals
Bioluminescence
Chickens
Food
Food Contamination - prevention & control
Food Microbiology
Genetic aspects
Health aspects
Image Processing, Computer-Assisted
Luminescence
Luminescent Proteins - biosynthesis
Luminescent Proteins - metabolism
Methods
Plasmids - genetics
Poultry Products - microbiology
Safety
Safety and security measures
Salmonella
Salmonella - classification
Salmonella - genetics
Salmonella - isolation & purification
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Summary:Salmonella can reside in healthy animals without the manifestation of any adverse effects on the carrier. If raw products of animal origin are not handled properly during processing or cooked to a proper temperature during preparation, salmonellosis can occur. In this research, we developed bioluminescent Salmonella strains that can be used for real-time monitoring of the pathogen's growth on food products. To accomplish this, twelve Salmonella strains from the broiler production continuum were transformed with the broad host range plasmid pAKlux1, and a chicken skin attachment model was developed. Salmonella strains carrying pAKlux1 constitutively expressed the luxCDABE operon and were therefore detectable using bioluminescence. Strains were characterized in terms of bioluminescence properties and plasmid stability. To assess the usefulness of bioluminescent Salmonella strains in food safety studies, we developed an attachment model using chicken skin. The effect of washing on attachment of Salmonella strains to chicken skin was tested using bioluminescent strains, which revealed the attachment properties of each strain. This study demonstrated that bioluminescence is a sensitive and effective tool to detect Salmonella on food products in real-time. Bioluminescence imaging is a promising technology that can be utilized to evaluate new food safety measures for reducing Salmonella contamination on food products.
ISSN:1471-2180
1471-2180
DOI:10.1186/1471-2180-8-10