Aseptic meningitis caused by torque teno virus in an infant: a case report

• Published in: Journal of medical case reports 2019-09, Vol.13 (1), p.302-302, Article 302
• Main Authors: Ikuta, Yoji, Oba, Kunihiro, Nai, Emina, Katori, Tatsuo, Hashino, Masanori, Inamine, Yuba, Matsunaga, Satoko, Yamaoka, Yutaro, Sekizuka, Tsuyoshi, Ryo, Akihide, Kuroda, Makoto
• Format: Article
• Language: English
• Subjects: Antibiotics; Antigens; Aseptic meningitis; Autoimmune diseases; Blood; Bone marrow; Case Report; Case reports; DNA Virus Infections - diagnosis; Genomes; Genomics; Health aspects; Hepatitis; High-Throughput Nucleotide Sequencing; High-throughput screening (Biochemical assaying); Humans; Immune response; Immunoglobulin M; Immunoglobulins; Infant; Infants; Infection; Infections; Infectious diseases; Male; Medical research; Meningitis; Meningitis, Aseptic - virology; Next generation sequencing; Nucleic acids; Pathogenesis; Polymerase Chain Reaction; Proteins; Risk factors; RNA; Technology; Torque teno virus; Torque teno virus - genetics; Torque teno virus - isolation & purification; TTV; Urine; Virus diseases; Viruses; Whole Genome Sequencing
• ISSN: 1752-1947; 1752-1947
• DOI: 10.1186/s13256-019-2233-2

Torque teno virus-induced aseptic meningitis has not been documented, although torque teno virus infections still remain under consideration for etiological agents. This study identified a torque teno virus sequence using next generation sequencing and immunoglobulin M response to the torque teno virus antigen, therefore, that would be a comprehensive diagnosis for torque teno virus infection. A 2-month-old Japanese boy was brought to our hospital because he was irritable, drowsy, and lethargic. He was admitted based on his test results which indicated the possibility of septic meningitis. He was started on treatment with high-dose antibiotics and steroids. On the third day of hospitalization, he became afebrile with improvement in his general status and was discharged on the sixth day. He had no developmental problems for up to 1 year after discharge. Metagenomic ribonucleic acid-Seq pathogen detection using next generation sequencing of a sample of his cerebrospinal fluid, which was collected at admission, revealed three short reads homologous to those in torque teno virus out of a total of 1,708,516 reads. This finding indicated that our patient was positive compared to the torque teno virus-negative cerebrospinal fluid samples (controls) from 13 other patients. The torque teno virus has been shown to have a whole genome sequence of 2810 nt by polymerase chain reaction. We prepared a recombinant GP2 antigen from torque teno virus and used it to study our patient's anti-torque teno virus immune response. An anti-GP2 serum immunoglobulin M response was detected, providing further supportive evidence of torque teno virus infection. This case speculates that torque teno virus-induced aseptic meningitis has a good course. New technologies like next generation sequencing can help in the identification of such cases, and an accumulation of future cases is expected.