Antioxidant potential of alkaloids and polyphenols of Viola canescens wall using in vitro and in silico approaches

• Published in: Frontiers in chemistry 2024, Vol.12, p.1379463-1379463
• Main Authors: Ahmad, Imtiaz, Huang, Pin-Jui, Malak, Nosheen, Khan, Adil, Asad, Fayaz, Chen, Chien-Chin
• Format: Article
• Language: English
• Subjects: antioxidants; flavonoids; in silico; in vitro; Viola canescencs
• ISSN: 2296-2646; 2296-2646
• DOI: 10.3389/fchem.2024.1379463

Wall, a plant renowned for its ethno-medical properties, was investigated in this study for its antioxidant potential based on its wide therapeutic applications in traditional healthcare systems. The study aimed to assess the antioxidant potential of the plant extract/fractions and to predict the active phytochemicals using computational techniques. Five fractions were obtained from the crude methanolic extract of and six concentrations (25, 50, 75, 100, 125, and 150 μg/mL) were prepared for each fraction. The antioxidant activity of these fractions was evaluated using the Tetraoxomolybdate (VI) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. docking studies and molecular dynamic simulations were conducted to further elucidate the molecular interactions underlying the antioxidant activity. The aqueous extract of exhibited significant antioxidant and free radical scavenging activity against DPPH. Additionally, the crude flavonoid extract demonstrated moderate activity with IC value of 57.863 μg/mL, indicating potent inhibition of cell growth. docking studies revealed a strong interaction between emetine and the aromatase protein, suggesting its potential as an antioxidant. The study findings highlight the antioxidant potential of extract, indicating its suitability as a source of natural antioxidants. These results suggest its potential application in pharmaceutical preparations aimed at harnessing antioxidant properties for therapeutic purposes.