Specific Monoclonal Antibodies against African Swine Fever Virus Protease pS273R Revealed a Novel and Conserved Antigenic Epitope

The African swine fever virus (ASFV) is a large enveloped DNA virus that causes a highly pathogenic hemorrhagic disease in both domestic pigs and wild boars. The ASFV genome contains a double-stranded DNA encoding more than 150 proteins. The ASFV possesses only one protease, pS273R, which is importa...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-08, Vol.25 (16), p.8906
Main Authors: Zhang, Jiajia, Zhang, Kaili, Sun, Shaohua, He, Ping, Deng, Dafu, Zhang, Pingping, Zheng, Wanglong, Chen, Nanhua, Zhu, Jianzhong
Format: Article
Language:English
Subjects:
African swine fever
African Swine Fever - immunology
African Swine Fever - virology
African swine fever virus
African Swine Fever Virus - immunology
Animals
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
Antigens, Viral - immunology
Cloning
diagnosis
Enzyme-Linked Immunosorbent Assay
epitope
Epitope Mapping
Epitopes - immunology
Hogs
Immunology
Infections
Mice
Mice, Inbred BALB C
Monoclonal antibodies
monoclonal antibody
Peptide Hydrolases - immunology
Peptide Hydrolases - metabolism
Peptides
Proteins
pS273R
Swine
Vaccines
Viral Proteins - immunology
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Summary:The African swine fever virus (ASFV) is a large enveloped DNA virus that causes a highly pathogenic hemorrhagic disease in both domestic pigs and wild boars. The ASFV genome contains a double-stranded DNA encoding more than 150 proteins. The ASFV possesses only one protease, pS273R, which is important for virion assembly and host immune evasion. Therefore, the specific monoclonal antibody (mAb) against pS273R is useful for ASFV research. Here, we generated two specific anti-pS273R mAbs named 2F3 and 3C2, both of which were successfully applied for ELISA, Western blotting, and immunofluorescence assays. Further, we showed that both 2F3 and 3C2 mAbs recognize a new epitope of N terminal 1-25 amino acids of pS273R protein, which is highly conserved across different ASFV strains including all genotype I and II strains. Based on the recognized epitope, an indirect ELISA was established and was effective in detecting antibodies during ASFV infection. To conclude, the specific pS273R mAbs and corresponding epitope identified will strongly promote ASFV serological diagnosis and vaccine research.
ISSN:1661-6596
1422-0067
1422-0067
DOI:10.3390/ijms25168906