PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech Republic

• Published in: Czech Journal of Food Sciences 2006-01, Vol.24 (3), p.127-132
• Main Authors: Maskova, E.(Vyzkumny Ustav Potravinarsky, Prague (Czech Republic)) E-mail:[e-mail], Paulickova, I.(Vyzkumny Ustav Potravinarsky, Prague (Czech Republic))
• Format: Article
• Language: English
• Subjects: ADN; ADULTERACION; ADULTERATION; Cheese; CONTROL DE CALIDAD; CONTROLE DE QUALITE; COW MILK; Cow's milk; Cytochrome; Cytochrome b; Cytochromes; Dairy products; Deoxyribonucleic acid; DNA; ELECTROFORESIS; ELECTROPHORESE; ELECTROPHORESIS; ENSAYO; EWE CHEESE; FROMAGE DE BREBIS; FROMAGE DE CHEVRE; GOAT CHEESE; Goats; LAIT DE VACHE; LECHE DE VACA; Milk; Mitochondria; PCR; Polymerase chain reaction; QUALITY CONTROLS; QUESO DE CABRA; QUESO DE OVEJA; Sheep; sheep cheese; TESTAGE; TESTING
• ISSN: 1212-1800; 1805-9317
• DOI: 10.17221/3307-cjfs

The aim of the study was to validate the PCR method exploiting the mitochondrial gene coding cytochrome b in order to detect cow's milk in goat and sheep cheeses. DNA was isolated from the cheeses using the isolation kit Invisorb Spin Food I. After electrophoresis, cow DNA was characterised by the fragment of the size of 274 bp, goat DNA by the fragment of 157 bp, and sheep DNA by the fragment of 331 bp. The detection limit of the PCR method described (1%) was determined with model samples made from pure goat cheese with a defined addition of cheese made from cow's milk. The method validated was applied in the analysis of 17 goat cheeses and 7 sheep cheeses obtained from retail trade. Products of Czech, Slovak, French, Dutch, and Italian origin were examined. The presence of undeclared cow's milk was detected in three kinds of goat cheese and in one kind of sheep cheese.