Prospective Study of the Detection of Bacterial Pathogens in Pediatric Clinical Specimens Using the Melting Temperature Mapping Method

The melting temperature ( ) mapping method is a novel technique that uses seven primer sets without sequencing to detect dominant bacteria. This method can identify pathogenic bacteria in adults within 3 h of blood collection without using conventional culture methods. However, no studies have exami...

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Bibliographic Details
Published in:Microbiology spectrum 2022-06, Vol.10 (3), p.e0019822-e0019822
Main Authors: Uejima, Yoji, Suganuma, Eisuke, Ohnishi, Takuma, Takei, Haruka, Furuichi, Mihoko, Sato, Satoshi, Kawano, Yutaka, Kitajima, Isao, Niimi, Hideki
Format: Article
Language:English
Subjects:
16S RNA
blood culture
bloodstream infections
clinical methods
Clinical Microbiology
pediatric infectious disease
rapid tests
Research Article
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Summary:The melting temperature ( ) mapping method is a novel technique that uses seven primer sets without sequencing to detect dominant bacteria. This method can identify pathogenic bacteria in adults within 3 h of blood collection without using conventional culture methods. However, no studies have examined whether pathogenic bacteria can be detected in clinical specimens from pediatric patients with bacterial infections. Here, we designed a new primer set for commercial use, constructed a database with more bacterial species, and examined the agreement rate of bacterial species . Moreover, we investigated whether our system could detect pathogenic bacteria from pediatric patients using the mapping method and compared the detection rates of the mapping and culture methods. A total of 256 pediatric clinical specimens from 156 patients (94 males and 62 females; median age, 2 years [
ISSN:2165-0497
2165-0497
DOI:10.1128/spectrum.00198-22