Glucosylceramide Plays a Role in Fungal Germination, Lipid Raft Organization and Biofilm Adhesion of the Pathogenic Fungus Scedosporium aurantiacum

Infections caused by species present a wide range of clinical manifestations, from superficial to disseminated, especially in immunocompromised patients. Glucosylceramides (GlcCer) are glycosphingolipids found on the fungal cell surface and play an important role in growth and pathogenicity processe...

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Published in:Journal of fungi (Basel) 2020-12, Vol.6 (4), p.345
Main Authors: Rochetti, Victor Pereira, Rollin-Pinheiro, Rodrigo, de Oliveira, Evely Bertulino, Xisto, Mariana Ingrid Dutra da Silva, Barreto-Bergter, Eliana
Format: Article
Language:English
Subjects:
Antibodies
biofilm
Biofilms
Cell surface
Chromatography
Conidia
Enzyme-linked immunosorbent assay
fungal growth
Fungi
Germination
Glucose
glucosylceramide
Glycosphingolipids
Immunocompromised hosts
Immunofluorescence
Lipid rafts
Lipids
Mass spectroscopy
Melanin
Metabolism
Pathogenicity
Polystyrene
Scedosporium
Sodium hydroxide
Virulence
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Summary:Infections caused by species present a wide range of clinical manifestations, from superficial to disseminated, especially in immunocompromised patients. Glucosylceramides (GlcCer) are glycosphingolipids found on the fungal cell surface and play an important role in growth and pathogenicity processes in different fungi. The present study aimed to evaluate the structure of GlcCer and its role during growth in two isolates. Purified GlcCer from both isolates were obtained and its chemical structure identified by mass spectrometry. Using ELISA and immunofluorescence techniques it was observed that germination and NaOH-treatment of conidia favor GlcCer exposure. Monoclonal anti-GlcCer antibody reduced germination when cultivated with the inhibitor of melanin synthesis tricyclazole and also reduced germ tube length of conidia, both cultivated or not with tricyclazole. It was also demonstrated that anti-GlcCer altered lipid rafts organization, as shown by using the fluorescent stain filipin, but did not affect the susceptibility of the cell surface to damaging agents. Anti-GlcCer reduced total biomass and viability in biofilms formed on polystyrene plates. In the presence of anti-GlcCer, germinated conidia and biofilms could not adhere to polystyrene with the same efficacy as control cells. These results highlight the relevance of GlcCer in growth processes of .
ISSN:2309-608X
2309-608X
DOI:10.3390/jof6040345