A high-density linkage map of the RN region in pigs

The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP marker...

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Bibliographic Details
Published in:Genetics selection evolution (Paris) 2000-05, Vol.32 (3), p.321-329, Article 321
Main Authors: Looft, C, Milan, D, Jeon, J T, Paul, S, Reinsch, N, Rogel-Gaillard, C, Rey, V, Amarger, V, Robic, A, Kalm, E, Chardon, P, Andersson, L
Format: Article
Language:English
Subjects:
Chromosome 15
Chromosomes
Cloning
Conformation
Fluorescence
Gene order
genetic mapping
Genetic markers
Genetics
Glycogen
Glycogens
Insulin-like growth factor-binding protein 5
Life Sciences
Linkage analysis
Meat
meat quality
Microsatellites
Physiological aspects
pig
Polymorphism
Quality
RN gene
Swine
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Summary:The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP markers (fluorescent single strand conformation polymorphism) and microsatellite markers. Subsequent multipoint linkage analysis revealed the order FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906. The gene order is identical to the previously reported porcine RH map of the same region. The described map will facilitate positional cloning of the RN gene.
ISSN:0999-193X
1297-9686
1297-9686
DOI:10.1186/1297-9686-32-3-321