A simple method to make, trap and deform a vesicle in a gel

We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the...

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Bibliographic Details
Published in:Scientific reports 2023-04, Vol.13 (1), p.5375-5375, Article 5375
Main Authors: Tapie, Pierre, Prevost, Alexis M., Montel, Lorraine, Pontani, Léa-Laetitia, Wandersman, Elie
Format: Article
Language:English
Subjects:
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639/301/923
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Humanities and Social Sciences
Hydration
Lipid bilayers
Lipids
Membrane proteins
Methods
multidisciplinary
Physics
Proteins
Science
Science (multidisciplinary)
Sediments
Vesicles
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Summary:We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the produced vesicle with fluorescence imaging and establish the presence and integrity of the lipid bilayer by the successful insertion of α -Hemolysin transmembrane proteins. Finally, we show that the vesicle can be easily mechanically deformed, non-intrusively, by indenting the surface of the gel.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-31996-9