New Insights on Nucleotide Sequence Variants and mRNA Levels of Candidate Genes Assessing Resistance/Susceptibility to Mastitis in Holstein and Montbéliarde Dairy Cows

A major factor in the propagation of an infectious disease is host genetics. In this study, 180 dairy cows (90 of each breed: Holstein and Montbéliarde) were used. Each breed's tested dairy cows were divided into two groups of comparable size (45 cows each), mastitis-free and mastitis-affected...

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Bibliographic Details
Published in:Veterinary sciences 2023-01, Vol.10 (1), p.35
Main Authors: Essa, Bothaina, Al-Sharif, Mona, Abdo, Mohamed, Fericean, Liana, Ateya, Ahmed
Format: Article
Language:English
Subjects:
Animal lactation
Animals
Association analysis
Breeding
candidate gene
CD46 antigen
Communicable diseases
Dairy cattle
Dairy industry
Disease resistance
Disease susceptibility
DNA sequencing
Ethylenediaminetetraacetic acid
Gene expression
Gene frequency
Genes
Genetic aspects
Genetic control
Genetic markers
Genomes
Genomics
Holstein
Infectious diseases
Inflammation
Jugular vein
Marker-assisted selection
Mastitis
Messenger RNA
Milk production
Montbéliarde
NF-κB protein
Nucleotide sequence
Nucleotide sequencing
Platelet-derived growth factor
Polymerase chain reaction
Single nucleotide polymorphisms
Single-nucleotide polymorphism
Susceptibility
Veterinary medicine
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Summary:A major factor in the propagation of an infectious disease is host genetics. In this study, 180 dairy cows (90 of each breed: Holstein and Montbéliarde) were used. Each breed's tested dairy cows were divided into two groups of comparable size (45 cows each), mastitis-free and mastitis-affected groups. Each cow's jugular vein was punctured to obtain blood samples for DNA and RNA extraction. In the examined Holstein and Montbéliarde dairy cows, single nucleotide polymorphisms (SNPs) related with mastitis resistance/susceptibility were found in the , , , , , , , , , , , , and genes. Chi-square analysis of identified SNPs revealed a significant difference in gene frequency between mastitic and healthy cows. Except for , mastitic dairy cows of two breeds had considerably higher mRNA levels of the examined genes than did healthy ones. Marker-assisted selection and monitoring of dairy cows' susceptibility to mastitis may be accomplished through the use of discovered SNPs and changes in the gene expression profile of the studied genes. These findings also point to a possible method for reducing mastitis in dairy cows through selective breeding of animals using genetic markers linked to an animal's ability to resist infection.
ISSN:2306-7381
2306-7381
DOI:10.3390/vetsci10010035