Effect of a Radical Mutation in Plastidic Starch Phosphorylase PHO1a on Potato Growth and Cold Stress Response

The plant response to stresses includes changes in starch metabolism regulated by a complex catalytic network, in which plastidic starch phosphorylase PHO1a is one of the key players. In this study, we used the CRISPR-Cas9 system to edit the PHO1a gene in four potato (Solanum tuberosum L.) cultivars...

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Bibliographic Details
Published in:Horticulturae 2022-08, Vol.8 (8), p.730
Main Authors: Nezhdanova, Anna V., Efremov, Gleb I., Slugina, Maria A., Kamionskaya, Anastasia M., Kochieva, Elena Z., Shchennikova, Anna V.
Format: Article
Language:English
Subjects:
Acclimatization (Plants)
Amylases
Biosynthesis
Catabolism
Cold
CRISPR
CRISPR-Cas9 editing
Cultivars
Domains
Editing
Environmental aspects
Enzymes
Gene expression
Genetic aspects
Growth (Plants)
Metabolism
Mutation
Phosphorylase
Phosphorylases
Physiological aspects
Plant resistance
Plasmids
potato Solanum tuberosum L
Potatoes
Proteins
Starch
starch metabolism
Starch phosphorylase
starch phosphorylase PHO1a
Transcription factors
Transgenic plants
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Summary:The plant response to stresses includes changes in starch metabolism regulated by a complex catalytic network, in which plastidic starch phosphorylase PHO1a is one of the key players. In this study, we used the CRISPR-Cas9 system to edit the PHO1a gene in four potato (Solanum tuberosum L.) cultivars, which resulted in the introduction of a radical mutation, G261V, into the PHO1a functional domain. The mutants had altered morphology and differed from wild-type plants in starch content in the roots and leaves. Exposure to cold stress revealed the differential response of parental and transgenic plants in terms of starch content and the expression of genes coding for β-amylases, amylase inhibitors, and stress-responsive MADS-domain transcription factors. These results suggest that the G261V mutation causes changes in the functional activity of PHO1a, which in turn affect the coordinated operation of starch catabolism enzymes both under normal and cold stress conditions, possibly through differential expression of MADS-domain transcription factors. Our results highlight a critical regulatory role of PHO1a in starch metabolism, root and shoot development, and stress response in potatoes.
ISSN:2311-7524
2311-7524
DOI:10.3390/horticulturae8080730