Hepatitis B virus infection and replication in human bone marrow mesenchymal stem cells

Hepatitis B virus (HBV) infection is a blood borne infectious disease that affects the liver. Human bone marrow mesenchymal stem cells (BMSCs) may serve as a cell source for adult stem cell transplantation in liver repair. However, the susceptibility of human BMSCs to HBV infection is poorly underst...

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Published in:Virology journal 2011-10, Vol.8 (1), p.486-486, Article 486
Main Authors: Ma, Ruiping, Xing, Quantai, Shao, Lihua, Wang, Dakun, Hao, Qingzhi, Li, Xia, Sai, Lintao, Ma, Lixian
Format: Article
Language:English
Subjects:
Adolescent
Adult
Bone marrow
Bone Marrow - virology
Care and treatment
Cells, Cultured
Chinese medicine
Culture Media - chemistry
Deoxyribonucleic acid
Diagnosis
DNA
DNA replication
DNA, Viral - analysis
DNA, Viral - genetics
Experiments
Genetic aspects
Health aspects
Hepatitis B
Hepatitis B e Antigens - metabolism
Hepatitis B virus
Hepatitis B virus - growth & development
Hospitals
Host Cell
Human bone marrow mesenchymal stem cell
Humans
Infection
Liver cancer
Medical research
Mesenchymal Stromal Cells - virology
Physiological aspects
Public health
Replication
Science
Stem cells
Studies
Young Adult
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cited_by cdi_FETCH-LOGICAL-b691t-946d86c4ba6ed0fb67326a54fa71b7094a70d6a2169e11e30a16cbdc129281f33
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creator Ma, Ruiping
Xing, Quantai
Shao, Lihua
Wang, Dakun
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Sai, Lintao
Ma, Lixian
description Hepatitis B virus (HBV) infection is a blood borne infectious disease that affects the liver. Human bone marrow mesenchymal stem cells (BMSCs) may serve as a cell source for adult stem cell transplantation in liver repair. However, the susceptibility of human BMSCs to HBV infection is poorly understood. The aim of this study was to investigate the infection and replication of HBV in cultures of human BMSCs. Human BMSCs were confirmed using flow cytometry. Intracellular HBV DNA was detected at d 2 after infection and maintained at relatively high levels from d 6 to d 12. The maximal level of intracellular HBV DNA was 9.37 × 105 copies/mL. The extracellular HBV DNA was observed from d 3 to d 15, and the levels ranged from 3.792 × 102 copies/mL to 4.067 × 105 copies/mL. HBsAg in the culture medium was detected from d 2 to d 16. HBeAg secretion was positive from d 5 to d 13. HBcAg constantly showed positive signals in approximately 7%-20% of BMSCs from 2 days after exposure. Intracellular HBV covalently closed circular DNA (cccDNA) could be detected as early as 2 days postinfection, and strong signals were obtained with increasing time. HBV can infect and replicate in human BMSCs. Human BMSCs may be a useful tool for investigating HBV life-cycle and the mechanism of initial virus-cell interactions.
doi_str_mv 10.1186/1743-422X-8-486
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Human bone marrow mesenchymal stem cells (BMSCs) may serve as a cell source for adult stem cell transplantation in liver repair. However, the susceptibility of human BMSCs to HBV infection is poorly understood. The aim of this study was to investigate the infection and replication of HBV in cultures of human BMSCs. Human BMSCs were confirmed using flow cytometry. Intracellular HBV DNA was detected at d 2 after infection and maintained at relatively high levels from d 6 to d 12. The maximal level of intracellular HBV DNA was 9.37 × 105 copies/mL. The extracellular HBV DNA was observed from d 3 to d 15, and the levels ranged from 3.792 × 102 copies/mL to 4.067 × 105 copies/mL. HBsAg in the culture medium was detected from d 2 to d 16. HBeAg secretion was positive from d 5 to d 13. HBcAg constantly showed positive signals in approximately 7%-20% of BMSCs from 2 days after exposure. 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Human bone marrow mesenchymal stem cells (BMSCs) may serve as a cell source for adult stem cell transplantation in liver repair. However, the susceptibility of human BMSCs to HBV infection is poorly understood. The aim of this study was to investigate the infection and replication of HBV in cultures of human BMSCs. Human BMSCs were confirmed using flow cytometry. Intracellular HBV DNA was detected at d 2 after infection and maintained at relatively high levels from d 6 to d 12. The maximal level of intracellular HBV DNA was 9.37 × 105 copies/mL. The extracellular HBV DNA was observed from d 3 to d 15, and the levels ranged from 3.792 × 102 copies/mL to 4.067 × 105 copies/mL. HBsAg in the culture medium was detected from d 2 to d 16. HBeAg secretion was positive from d 5 to d 13. HBcAg constantly showed positive signals in approximately 7%-20% of BMSCs from 2 days after exposure. Intracellular HBV covalently closed circular DNA (cccDNA) could be detected as early as 2 days postinfection, and strong signals were obtained with increasing time. HBV can infect and replicate in human BMSCs. Human BMSCs may be a useful tool for investigating HBV life-cycle and the mechanism of initial virus-cell interactions.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>22035170</pmid><doi>10.1186/1743-422X-8-486</doi><oa>free_for_read</oa></addata></record>
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ispartof Virology journal, 2011-10, Vol.8 (1), p.486-486, Article 486
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subjects Adolescent
Adult
Bone marrow
Bone Marrow - virology
Care and treatment
Cells, Cultured
Chinese medicine
Culture Media - chemistry
Deoxyribonucleic acid
Diagnosis
DNA
DNA replication
DNA, Viral - analysis
DNA, Viral - genetics
Experiments
Genetic aspects
Health aspects
Hepatitis B
Hepatitis B e Antigens - metabolism
Hepatitis B virus
Hepatitis B virus - growth & development
Hospitals
Host Cell
Human bone marrow mesenchymal stem cell
Humans
Infection
Liver cancer
Medical research
Mesenchymal Stromal Cells - virology
Physiological aspects
Public health
Replication
Science
Stem cells
Studies
Young Adult
title Hepatitis B virus infection and replication in human bone marrow mesenchymal stem cells
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