Mass spectrometry analysis of circulating breast cancer cells from a Xenograft mouse model

Circulating tumor cells (CTCs) are precursors of metastasis in various cancer types. Many aspects regarding CTC biology remain poorly understood. Here, we describe mass spectrometric analysis of CTCs from a breast cancer xenograft mouse model, including procedures comprising CTC enrichment, separati...

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Bibliographic Details
Published in:STAR protocols 2021-06, Vol.2 (2), p.100480-100480, Article 100480
Main Authors: Donato, Cinzia, Buczak, Katarzyna, Schmidt, Alexander, Aceto, Nicola
Format: Article
Language:English
Subjects:
Animals
Breast Neoplasms - chemistry
Cancer
Cell Biology
Cell isolation
Female
Flow Cytometry/Mass Cytometry
Heterografts
Humans
Mass Spectrometry
Mass Spectrometry - methods
Mice
Model Organisms
Neoplasm Transplantation
Neoplastic Cells, Circulating - chemistry
Proteome - analysis
Proteome - chemistry
Proteomics
Protocol
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Summary:Circulating tumor cells (CTCs) are precursors of metastasis in various cancer types. Many aspects regarding CTC biology remain poorly understood. Here, we describe mass spectrometric analysis of CTCs from a breast cancer xenograft mouse model, including procedures comprising CTC enrichment, separation of different CTC subpopulations, and their quantitative proteomic assessment. This protocol aims to facilitate the identification of protein content dynamics in human CTCs that are physiologically shed from tumor-bearing xenografts, providing a framework for investigating metastasis biology. For complete details on the use and execution of this protocol, please refer to Donato et al. (2020). [Display omitted] •Method for mass spectrometry analysis of circulating tumor cells from xenografts•Procedure to stain and separate subpopulations of circulating tumor cells•Methods to perform data analysis from mass spectrometry of circulating tumor cells Circulating tumor cells (CTCs) are precursors of metastasis in various cancer types. Many aspects regarding CTC biology remain poorly understood. Here, we describe mass spectrometric analysis of CTCs from a breast cancer xenograft mouse model, including procedures comprising CTC enrichment, separation of different CTC subpopulations, and their quantitative proteomic assessment. This protocol aims to facilitate the identification of protein content dynamics in human CTCs that are physiologically shed from tumor-bearing xenografts, providing a framework for investigating metastasis biology.
ISSN:2666-1667
2666-1667
DOI:10.1016/j.xpro.2021.100480