Phenotypic and Proteomic Characteristics of Human Dental Pulp Derived Mesenchymal Stem Cells from a Natal, an Exfoliated Deciduous, and an Impacted Third Molar Tooth

The level of heterogeneity among the isolated stem cells makes them less valuable for clinical use. The purpose of this study was to understand the level of heterogeneity among human dental pulp derived mesenchymal stem cells by using basic cell biology and proteomic approaches. The cells were isola...

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Published in:Stem Cells International 2014-01, Vol.2014 (2014), p.248-266
Main Authors: Gacar, Gulcin, Duruksu, Gokhan, Aksoy, Ayca, Kasap, Murat, Akpinar, Gurler, Karaöz, Erdal
Format: Article
Language:English
Subjects:
Apoptosis
Biomedical research
Bone marrow
Cellular proteins
Dental pulp
Flow cytometry
Gene expression
Growth
Metabolism
Morphology
Phenotype
Properties
Protein expression
Proteins
Proteomics
Stem cell research
Stem cells
Studies
Teeth
Telomerase
Transplants & implants
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description The level of heterogeneity among the isolated stem cells makes them less valuable for clinical use. The purpose of this study was to understand the level of heterogeneity among human dental pulp derived mesenchymal stem cells by using basic cell biology and proteomic approaches. The cells were isolated from a natal (NDPSCs), an exfoliated deciduous (stem cells from human exfoliated deciduous (SHED)), and an impacted third molar (DPSCs) tooth of three different donors. All three stem cells displayed similar features related to morphology, proliferation rates, expression of various cell surface markers, and differentiation potentials into adipocytes, osteocytes, and chondrocytes. Furthermore, using 2DE approach coupled with MALDI-TOF/TOF, we have generated a common 2DE profile for all three stem cells. We found that 62.3 ± 7 % of the protein spots were conserved among the three mesenchymal stem cell lines. Sixty-one of these conserved spots were identified by MALDI-TOF/TOF analysis. Classification of the identified proteins based on biological function revealed that structurally important proteins and proteins that are involved in protein folding machinery are predominantly expressed by all three stem cell lines. Some of these proteins may hold importance in understanding specific properties of human dental pulp derived mesenchymal stem cells.
doi_str_mv 10.1155/2014/457059
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source Wiley Online Library Open Access; Publicly Available Content Database; PubMed Central
subjects Apoptosis
Biomedical research
Bone marrow
Cellular proteins
Dental pulp
Flow cytometry
Gene expression
Growth
Metabolism
Morphology
Phenotype
Properties
Protein expression
Proteins
Proteomics
Stem cell research
Stem cells
Studies
Teeth
Telomerase
Transplants & implants
title Phenotypic and Proteomic Characteristics of Human Dental Pulp Derived Mesenchymal Stem Cells from a Natal, an Exfoliated Deciduous, and an Impacted Third Molar Tooth
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