Diversity of Sinorhizobium (Ensifer) meliloti Bacteriophages in the Rhizosphere of Medicago marina : Myoviruses, Filamentous and N4-Like Podovirus

Using different strains as hosts, we isolated eight new virulent phages from the rhizosphere of the coastal legume . Half of the isolated phages showed a very narrow host range while the other half exhibited a wider host range within the strains tested. Electron microscopy studies showed that phages...

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Bibliographic Details
Published in:Frontiers in microbiology 2020-01, Vol.11, p.22-22
Main Authors: Cubo, María Teresa, Alías-Villegas, Cynthia, Balsanelli, Eduardo, Mesa, Dany, de Souza, Emanuel, Espuny, María Rosario
Format: Article
Language:English
Subjects:
bacteriophages
filamentous phage
Microbiology
N4-like phage
P_ort11
Podoviridae
Sinorhizobium/Ensifer meliloti
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Summary:Using different strains as hosts, we isolated eight new virulent phages from the rhizosphere of the coastal legume . Half of the isolated phages showed a very narrow host range while the other half exhibited a wider host range within the strains tested. Electron microscopy studies showed that phages M_ort18, M_sf1.2, and M_sf3.33 belonged to the family with feature long, contractile tails and icosaedral head. Phages I_sf3.21 and I_sf3.10T appeared to have filamentous shape and produced turbid plaques, which is a characteristic of phages from the family. Phage P_ort11 is a member of the , with an icosahedral head and a short tail and was selected for further characterization and genome sequencing. P_ort11 contained linear, double-stranded DNA with a length of 75239 bp and 103 putative open reading frames. BLASTP analysis revealed strong similarities to phage N4 and other N4-like phages. This is the first report of filamentous and N4-like phages that infect
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2020.00022